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栀子叶斑病及其病原的鉴定
引用本文:黄学顺,付艳平,秦乐业,李勋远,向青锋,曾国柱,姜道宏.栀子叶斑病及其病原的鉴定[J].华中农业大学学报,2006,25(1):21-25.
作者姓名:黄学顺  付艳平  秦乐业  李勋远  向青锋  曾国柱  姜道宏
作者单位:1. 湖北省长阳县都镇湾镇农技站,长阳,443509;华中农业大学植物科学技术学院,武汉,430070
2. 华中农业大学植物科学技术学院,武汉,430070
3. 湖北省长阳县农业局,长阳,443500
4. 湖北省长阳县都镇湾镇农技站,长阳,443509
5. 华中农业大学植物科学技术学院,武汉,430070;华中农业大学农业微生物学国家重点实验室,武汉,430070
基金项目:国家星火计划项目(2004EA760007)资助
摘    要:首次报道在栀子(Gardenia jasminoides)上存在由拟盘多毛孢属真菌引致的叶斑病。从湖北省长阳县采集到栀子叶斑病病害标本,并利用柯赫氏法则证实该病由真菌GJ-1菌株所引致;根据GJ-1菌株的形态学特征和ITS序列的分析、比较,证实GJ-1菌株属于拟盘多毛孢属(PestalotiopsisSteyaert)真菌;GJ-1菌株的ITS序列与P.gracilis的ITS同源性达100%,因此推定它属于P.gracilis。

关 键 词:栀子  叶斑病  拟盘多毛孢属  核糖体DNA-ITS
文章编号:1000-2421(2006)01-0021-05
收稿时间:03 17 2005 12:00AM
修稿时间:2005-03-172005-08-09

The Leaf Spot on Gardenia jasminoides and Identification of Its Pathogen
HUANG Xue-shun,FU Yan-ping,QIN Le-ye,LI Xun-yuan,XIANG Qing-feng,ZENG Guo-zhu,JIANG Dao-hong.The Leaf Spot on Gardenia jasminoides and Identification of Its Pathogen[J].Journal of Huazhong Agricultural University,2006,25(1):21-25.
Authors:HUANG Xue-shun  FU Yan-ping  QIN Le-ye  LI Xun-yuan  XIANG Qing-feng  ZENG Guo-zhu  JIANG Dao-hong
Institution:1 . Extension Station of Agricultural Science and Technology at Duzhenwan, Hubei Province, Changyang 443509, China; 2. College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; 3.Agricultural Office of Changyang County , Hubei Province ,Changyang 443500,China; 4. State Key Lab of Agriculture Microbiology, Huazhong Agricultural University , Wuhan 430070, China
Abstract:Diseased leaves of G.jasminoides with spot symptom were collected from Changyang County,Hubei Province,and a fungal stain GJ-1 was isolated and proved to be the pathogen of the leaf spot disease through a procedure of Koch's postulation.Based on the morphological characteristics of conidia and the sequence of the internal transcribed spacer,GJ-1 was identified as a species of Pestalotiopsis Steyaert.The ITS sequence of GJ-1 showed 100% identity to that of P.gracilis,therefore GJ-1 belong to P.gracilis.It is the first report that Pestalotiopsis can cause spot disease on G.jasminoides.
Keywords:Gardenia jasminoides  leaf spot  Pestalotiopsis gracilis  rDNA-ITS
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