解钾菌解钾效率检测方法的比较

以中慢生根瘤菌S-15和类芽胞杆菌S-17作为供试菌株,采用细胞破碎、NH4OAc浸提、H2O2溶液消煮及不作任何前处理等4种方法,利用火焰光度计检测解钾菌发酵液中K+含量,并计算解钾菌在培养基中的解钾效率。结果表明,配制的3种钾系列标准溶液所绘制的钾标准曲线较为接近,R2分别高达0.994 4、0.9997、0.999 8。采用H2O2消煮后所测得的K+浓度最高,2株解钾菌的解钾效率分别达到101.1%、125.1%,与其他处理组之间有显著性差异。用H2O2溶液处理所得到的解钾率更能真实反映解钾菌的解钾作用。

Comparing different methods of detecting potassium solubilizing efficiency with potassium solubilizing bacteria

Using Mesorhizobium sp.S-15 and Paenibacillus sp.S-17 as experimental strains.After cell disruption,NH 4 OAc extraction,H 2 O 2 solution digestion or without any pre-treatment,K+ content in the fermentation broth of potassium solubilizing bacteria was determined with flame photometer.The ef-ficiency of K solubilizing by potassium solubilizing bacteria in the culture medium was calculated.The re-sults showed that the standard curves of the three kinds of K series of standard solutions were similar with the R 2 values of 0.994 4,0.999 7 and 0.999 8,respectively.After digested by H 2 O 2 solution,concen-tration of K+ was detected.The efficiency of two strains was 101.1% and 125.1%,significantly higher than that of other groups.The efficiency of potassium solubilizing detected after H 2 O 2 digestion well re-flected potassium solubilization of bacteria.