饲料中添加芽孢杆菌对草鱼表观消化率及消化酶活性的影响

在草鱼(Ctenopharyngodon idellus)饲料中分别添加0(Ⅰ组)、1×108(Ⅱ组)、3×108(Ⅲ组)、5×108cfu/g(Ⅳ组)芽孢杆菌(Bacillus spp.)持续投喂草鱼,收集粪便测定草鱼对各营养物质的表观消化率和消化酶活性。结果表明:投喂30d后,Ⅱ、Ⅲ组干物质、粗蛋白、粗脂肪表观消化率和Ⅳ组粗蛋白、粗脂肪表观消化率均显著高于Ⅰ组(对照组),其中Ⅱ组的表观消化率最高,且芽孢杆菌添加量与营养物质表观消化率最符合一元二次曲线方程。Ⅱ组中肠和Ⅱ、Ⅲ组肝胰脏蛋白酶活性均显著提高;Ⅱ组的前肠和后肠淀粉酶活性显著高于对照组;Ⅱ、Ⅲ组后肠脂肪酶活性均显著高于对照组。投喂60d后,消化酶活性也有相似的结果。双因子方差分析表明:芽孢杆菌的添加量对中肠、后肠和肝胰脏蛋白酶活性,前肠、中肠和后肠淀粉酶活性,中肠和后肠脂肪酶活性均有显著影响;投喂时间对肝胰脏蛋白酶、淀粉酶活性有显著影响。

Dietary supplement of Bacillus spp.affected apparent digestibility,digestive enzyme activity of Ctenopharyngodon idellus

Ctenopharyngodon idellus were fed with basal diet supplemented with 0(Ⅰ),1×108(Ⅱ),3×108(Ⅲ),5×108 cfu/g(Ⅳ) Bacillus spp.respectively for 30 days.Feces was collected to detect apparent digestibility of feed nutrients.The apparent digestibility of dry matter,crude protein and crude lipid in Ⅰ group was significantly lower than the other treatment groups(P<0.05).Quadratic curve equation was the best to fit the relationship between dosages of Bacillus spp.and apparent digestibility of nutrients.Protease activity of the mid-gut in Ⅱgroup and the hepatopancreas inⅡ,Ⅲ groups increased significantly compared with Ⅰ group(P<0.05).Amylase activity of the fore-gut and hind-gut in Ⅱgroup increased significantly compared with Ⅰ group(P<0.05).Lipase activity of the hind-gut in Ⅱ,Ⅲ group increased significantly compared with Ⅰ group(P<0.05).After feeding for 60 days,the similar results appeared.Two-way analysis of variance showed that protease activity of the mid-gut,hind-gut and the hepatopancreas,amylase activity of the fore-gut,mid-gut and hind-gut,lipase activity of the mid-gut and hind-gut were all significantly affected by the additional doseages of Bacillus spp.(P<0.05).Protease activity and amylase activity of the hepatopancreas were significantly affected by feeding days(P<0.05).