HMC毒素诱导玉米专效寄主原生质体细胞凋亡的检测

以2对同核异质玉米B37和Mo17叶片的原生质体为试材,并将原生质体随机分为4组,HMC毒素质量浓度分别为0、50、100、150μg/mL,处理时间分别为1、3、6h。采用吖啶橙(AO)与溴化乙啶(EB)荧光复染的方法进行检测,观察处理后原生质体的凋亡特征及细胞凋亡率的变化趋势。结果表明:HMC毒素处理玉米原生质体后发生细胞凋亡,并出现凋亡小体、染色质边集或环状染色质等凋亡特征;经不同浓度HMC毒素处理B37和Mo17玉米原生质体,CB37的最大凋亡率为10.80%,NB37为4.90%,CMo17为21.00%,NMo17为8.83%;HMC毒素对2种基因型的C、N细胞质所测结果一致,均为C细胞质对毒素敏感,先出现细胞凋亡,N细胞质出现细胞凋亡的时间相对较晚;C、N之间的细胞凋亡率差异显著,且前者高于后者。当毒素浓度不变时,细胞凋亡率随毒素处理时间的延长而递增;当处理时间不变时,细胞凋亡率随毒素浓度的增加而上升。

Detection of apoptosis in host protoplasts induced by HMC-toxin

Apoptosis in protoplasts from homokaryon maize B37(CB37,NB37) and Mo17(CMo17,NMo17) inbred lines treated by HMC-toxin were studied.Homokaryon maize B37 and Mo17 inbred lines were tested and the protoplasts may be stained by AO and EB.The protoplasts were divided into 4 groups.3 groups were treated by 0,50,100,150 μg/mL of HMC-toxin as the experimental groups,and the induced time were 1,3,6 h to study the feature of apoptotic and the rate of apoptosis.The apoptotic features such as apoptotic bodies,chromatin margination or ring chromatin were found after the treatment.When treated by HMC-toxin with different concentrations,the apoptosis rates of CB37,NB37,CMo17 and NMo17 maize protoplasts were 10.80%,4.90%,21.00%,and 8.83%.According to the above study,a conclusion can be reached as follows: apoptosis in protoplasts from homokaryon maize B37 were the same as that of Mo17;CB37 and CMo17 were more sensitive to HMC-toxin than NB37 and NMo17,respectively;when treated by the same concentration of HMC-toxin,the apoptosis rates of CB37 and CMo17 maize protoplasts were significantly higher than NB37 and NMo17 respectively;the rates of apoptosis were significantly different between C and N,and the former was significantly higher than the latter.In addition,the apoptosis rate increased when the treatment time and the concentration increased.