| 番茄抗南方根结线虫种质材料NBS-LRR类抗病基因的检测 |
| |
| 引用本文： | 陆秀红,黄金玲,刘志明,秦碧霞,乔丽娅.番茄抗南方根结线虫种质材料NBS-LRR类抗病基因的检测[J].华中农业大学学报,2012,31(2):208-211. |
| |
| 作者姓名： | 陆秀红 黄金玲 刘志明 秦碧霞 乔丽娅 |
| |
| 作者单位： | 广西农业科学院植物保护研究所，南宁 530007；广西大学农学院，南宁 530005；广西作物遗传改良生物技术重点开放实验室，南宁 530007;广西农业科学院植物保护研究所，南宁 530007；广西大学农学院，南宁 530005;广西农业科学院植物保护研究所，南宁 530007；广西大学农学院，南宁 530005;广西农业科学院植物保护研究所，南宁 530007;广西大学农学院，南宁 530005 |
| |
| 基金项目： | 广西自然科学基金项目（2010GXNSFB013035）和广西农科院基本科研业务项目（200829基） |
| |
| 摘 要： | 采用盆栽病土接种法,鉴定58份番茄(Solanum lycopersicum)种质材料对南方根结线虫(Meloido-gyne incognita)的抗性。结果表明:供试的58份番茄种质材料对南方根结线虫的抗病性存在明显差异,其中高抗材料1份,抗病材料3份,感病材料3份,高感材料51份;根据已知NBS-LRR类抗病基因NBS区域的保守系列,设计简并引物,对抗性材料基因组进行体外扩增,获得了约500bp的预期片段。
|
| 关 键 词： | 番茄 南方根结线虫 抗性 检测 |
| 收稿时间： | 2011/6/20 0:00:00 |
Detection of NBS-LRR type disease resistance gene in the resistance cultivars of tomato to Meloidogyne incognita |
| |
| LU Xiu-hong,HUANG Jin-ling,LIU Zhi-ming,QIN Bi-xia,QIAO Li-ya.Detection of NBS-LRR type disease resistance gene in the resistance cultivars of tomato to Meloidogyne incognita[J].Journal of Huazhong Agricultural University,2012,31(2):208-211. |
| |
| Authors: | LU Xiu-hong HUANG Jin-ling LIU Zhi-ming QIN Bi-xia QIAO Li-ya |
| |
| Institution: | 1.Institute of Plant Protection,Guangxi Academy of Agricultural Sciences, Nanning 530007,China; 2.College of Agronomy,Guangxi University,Nanning 530005,China; 3.Guangxi Crop Genetic Improvement and Biotechnology Laboratory,Nanning 530007,China |
| |
| Abstract: | The resistance of 58 tomato Solanum lycopersicum materials to Meloidogyne incognita were studied by transplanting the tomatoes to disease soil in the pot.There were significant differences between 58 cultivars.The results showed that 1 cultivar was highly resistant,3 were resistant,3 were susceptible,51 were highly susceptible to Meloidogyne incognita.Degenerate oligonucleotide primers were designed according to the conserved NBS motifs among some disease-resistant genes.These primers were used for the amplification of the disease-resistant gene analog(RGA) from the genome of resistance cultivars by polymerase chain reaction(PCR),and about 500 bp-long PCR product was obtained. |
| |
| Keywords: | tomato Meloidogyne incognita resistance detection |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《华中农业大学学报》浏览原始摘要信息 |
| 点击此处可从《华中农业大学学报》下载免费的PDF全文 |
|
