星星草PutNaKR3基因的克隆及其耐逆性分析

以NaCl胁迫星星草(Puccinellia tenuiflora)cDNA酵母文库为基础,在星星草中筛选和鉴定PutNaKR3基因,对PutNaKR3基因及其编码的氨基酸序列进行生物信息学、组织特异性表达和非生物胁迫应答表达分析。结果表明:星星草PutNaKR3基因具有组织表达特异性,在根和叶中的表达量明显高于其他组织;过表达PutNaKR3基因可增强酵母菌株在盐、碱、氧化和渗透胁迫下的生存能力,同时可提高酵母在部分金属胁迫下的适应能力。

Cloning a PutNaKR3 gene in Puccinellia tenuiflora and analyzing its stress tolerance

A PutNaKR3 gene was screened and identified from the cDNA library of Puccinellia tenuiflora under NaCl treatment. Bioinformatics, tissue specific expression and abiotic stress response expression of the PutNaKR3 gene and its encoded amino acid sequence were performed. The results showed that PutNaKR3 gene in Puccinellia tenuiflora had a tissue expression specificity, and its expression in roots and leaves was significantly higher than that in other tissues. Over expression of PutNaKR3 gene enhanced the viability of yeast strain under salt, alkali, oxidation and osmotic stress, and improved the adaptability of yeast under partial metal stress. It is indicated that the PutNaKR3 gene plays a certain role in plant stress response, especially in enhancing plant salt tolerance.