| 杨树肉桂醇脱氢酶基因序列和表达模式分析及CAD4酶活性检测 |
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| 引用本文： | 任珊珊,赵艳玲,白 华,蒋湘宁,盖 颖.杨树肉桂醇脱氢酶基因序列和表达模式分析及CAD4酶活性检测[J].华中农业大学学报,2011,30(5):578-584. |
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| 作者姓名： | 任珊珊 赵艳玲 白 华 蒋湘宁 盖 颖 |
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| 作者单位： | 1. 北京林业大学生物科学与技术学院,北京,100083
2. 华侨大学化工学院,厦门,361021 |
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| 基金项目： | 国家自然科学基金重点项目(NSF30630053); 福建省科技计划重点项目(2010N0018); 北京林业大学新进教师科研启动基金项目(2010BLX03); 中国博士后科学基金项目(20090450015) |
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| 摘 要： | 应用生物信息学方法从杨树基因组数据库中筛选出19个杨树肉桂醇脱氢酶基因,它们聚为3类.序列分析表明,杨树CAD基因家族主要通过全基因组加倍和串联复制的方式进行扩张.杨树和拟南芥CAD家族共6对旁系同源基因,在较强的净化选择压力下进化.19个基因在杨树不同组织中均有表达,表达量较高的是PoptrCAD17、PoptrCA...
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| 关 键 词： | 杨树 肉桂醇脱氢酶(CAD) CAD基因家族 基因重复 组织表达 酶活检测 |
| 收稿时间： | 2011/5/26 0:00:00 |
Sequences and expression pattern of cinnamyl alcohol dehydrogenase gene and over-expression and detection of CAD4 enzymatic activity of poplar in vitro |
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| REN Shan-shan,ZHAO Yan-ling,BAI Hua,JIANG Xiang-ning,GAI Ying.Sequences and expression pattern of cinnamyl alcohol dehydrogenase gene and over-expression and detection of CAD4 enzymatic activity of poplar in vitro[J].Journal of Huazhong Agricultural University,2011,30(5):578-584. |
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| Authors: | REN Shan-shan ZHAO Yan-ling BAI Hua JIANG Xiang-ning GAI Ying |
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| Institution: | REN Shan-shan1 ZHAO Yan-ling2 BAI Hua1 JIANG Xiang-ning1 GAI Ying1 1.College of Biology and Biotechnology,Beijing Forestry University,Beijing 100083,China,2.College of Chemical Engineering,Huaqiao University,Xiamen 361021 |
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| Abstract: | The 19 CAD genes of poplar screened by bioinformatics are clustered into 3 groups.Analysis of their sequences showed that the expansion of CAD gene family in Populus was resulted from a recent whole-genome doubling and tandem replication.There are 6 paralogs in CAD gene family of Populus and Arabidopsis evolved under the purifying selection.The 19 CAD genes are expressed in all tissues of Populus.PoptrCAD17,PoptrCAD4,PoptrCAD10 and PoptrCAD7 expressed highly while the other CAD genes expressed at a lower le... |
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| Keywords: | poplar cinnamyl alcohol dehydrogenase(CAD) CAD gene family gene duplication tissue expression enzyme activity detection |
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