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PEG胁迫对香石竹幼苗的影响及香石竹抗旱性评价
引用本文:孔维龙,闫艳丽,杨绍宗,但乃震,王禹璐,包满珠,傅小鹏.PEG胁迫对香石竹幼苗的影响及香石竹抗旱性评价[J].华中农业大学学报,2017,36(6):17-23.
作者姓名:孔维龙  闫艳丽  杨绍宗  但乃震  王禹璐  包满珠  傅小鹏
作者单位:华中农业大学园艺林学学院/园艺植物生物学教育部重点实验室,武汉,430070
基金项目:国家自然科学基金(31000918); 中央高校基本科研业务费专项(2662015PY052; 2662016PY041)
摘    要:为探究干旱胁迫对香石竹幼苗的影响,鉴定香石竹各品种的抗旱能力,以9个香石竹品种为研究对象,采用PEG 6000模拟干旱胁迫,分析叶片相对含水量(RWC)、超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、可溶性蛋白、丙二醛(MDA)、脯氨酸(Pro)等7个生理生化指标的变化,并基于这7个理化指标的抗旱指数,对香石竹各品种进行主成分分析及综合评价。结果表明:9个香石竹品种在水分胁迫处理时各品种各指标存在显著差异(P0.05)。将7个抗旱指标用主成分分析法归纳成3个主成分,累积贡献率为84.46%,利用隶属函数法评定的抗旱性强弱顺序为马斯特、苔丝、皇族、紫罗兰、火焰、粉恋、锦葵钛合金、粉黛、自由。

关 键 词:香石竹  干旱胁迫  PEG  6000  主成分分析  综合评价
收稿时间:2017/6/25 0:00:00

Effects of PEG stress on carnation seedlings and evaluating drought resistance of different carnation
KONG Weilong,YAN Yanli,YANG Shaozong,DAN Naizhen,WANG Yulu,BAO Manzhu,FU Xiaopeng.Effects of PEG stress on carnation seedlings and evaluating drought resistance of different carnation[J].Journal of Huazhong Agricultural University,2017,36(6):17-23.
Authors:KONG Weilong  YAN Yanli  YANG Shaozong  DAN Naizhen  WANG Yulu  BAO Manzhu  FU Xiaopeng
Abstract:The relative water content (RWC),superoxide dismutase (SOD),peroxidase (POD),catalase (CAT),soluble protein,malondialdehyde (MDA) and proline (Pro) under drought stress at 20% PEG 6000 concentration were studied to explore the effect of drought stress on carnation seedlings and evaluate the drought resistance of 9 varieties of carnation.Based on the drought resistance index of these seven physical and chemical indexes,the principal component analysis and comprehensive evaluation of all varieties of carnation were carried out.The results showed that there were significant differences among the indexes of 9 carnation cultivars under water stress (P<0.05).Seven drought resistance indexes were classified into four principal components by principal component analysis,with the cumulative contribution rate of 84.46%.The drought resistance evaluated by the membership function method is in the order of ‘Master’,‘Tess’,‘Royal’,‘Violet’,‘Flame’,‘Pink Love’,‘Mallow Titanium Alloy’,‘Pink Superior’,‘Freedom’.
Keywords:carnation  drought stress  PEG 6000  principal component analysis  comprehensive evaluation
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