| 电场诱导融合再生两个柑桔种间体细胞杂种 |
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| 引用本文： | 余桂红,邓秀新.电场诱导融合再生两个柑桔种间体细胞杂种[J].华中农业大学学报,2000,19(3):268-273. |
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| 作者姓名： | 余桂红 邓秀新 |
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| 作者单位： | 华中农业大学作物遗传改良国家重点实验室,武汉,430070 |
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| 基金项目： | SupportedbytheNationalNaturalScienceFoundationofChina (NSFC) |
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| 摘 要： | 通过电场诱导融合方法分别获得了续荷尔脐橙与粗柠檬、纽荷尔脐橙与夔柑两个组合的体细胞杂种。纽荷尔脐橙＋粗柠檬级合获得了４５棵再生植株。对其中的３０棵株进行染色体检查，结果表明，全为四部体，２ｎ＝４ｘ＝３６。过氧化物同工酶及ＲＡＰＤ分析表明再生植株为体细胞杂种。续荷尔脐橙＋夔柑组合获得了２５株再生植株。染色体检查结果表明，全为四倍体，２ｎ＝４ｘ＝３６。ＲＡＰＤ分析表明再生植株为体细胞杂种。
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| 关 键 词： | 柑桔 电融合 体细胞杂种 HAPD分析 品种改良 |
Two Interspecific Somatic Hybrids of Citrus Produced via Protoplast Electrofusion |
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| Yu Guihong,Deng Xiuxin.Two Interspecific Somatic Hybrids of Citrus Produced via Protoplast Electrofusion[J].Journal of Huazhong Agricultural University,2000,19(3):268-273. |
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| Authors: | Yu Guihong Deng Xiuxin |
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| Abstract: | Protoplasts of the embryogenic callus of Newhall navel orange (Citrus sinensis) were electro-fused with mesophyll protoplast s of Rough Lemon (C. jambhiri) and Kuigan tangelo (C. reticulata × C. grandisi ) respectively. 45 and 25 plants regenerated from these 2 combinations respectively. Chromosome cou nts of 30 plants of the combination of Newhall navel orange +Rough lemon, and 25 of Newhall + Kuigan showed that all of them were tetraploids (2n=4x=36). Peroxi dase isozyme and RAPD analysis verified that the tetraploid plants of Newhall + Rough lemon were somatic hybrids. RAPD analysis with primer OPA14 and OPA08 toge ther verified that all of the tetraploids derived from Newhall+ Kuigan were also somatic hybrid plants. |
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| Keywords: | citrus protoplast electro fusion somatic hybrid RAPD analysis genetic improvement |
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