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拟南芥Ran小GTP结合蛋白在细胞有丝分裂中的定位
引用本文:马立安,江涛,张忠明.拟南芥Ran小GTP结合蛋白在细胞有丝分裂中的定位[J].华中农业大学学报,2008,27(6).
作者姓名:马立安  江涛  张忠明
作者单位:1. 长江大学生命科学学院,荆州,434025;华中农业大学农业微生物学国家重点实验室,武汉,430070
2. 长江大学动物科学学院,荆州,434025
3. 华中农业大学农业微生物学国家重点实验室,武汉,430070
基金项目:国家自然科学基金项目 , 美国国家科学基金项目  
摘    要:分别采用RT-PCR和直接荧光免疫方法分析Ran2 mRNA在拟南芥不同组织器官中的表达及Ran2的亚细胞定位。结果表明,Ran2基因在拟南芥根、茎、叶和花中均有表达;Ran2蛋白在细胞分裂间期主要定位于核膜周边,在后期定位于赤道板上和纺锤体上,末期又回到子细胞核膜周边。

关 键 词:拟南芥Ran2小GTP结合蛋白  FITC-anti-Ran2抗体  亚细胞定位

The Localization of Arabidopsis thaliana Ran Small GTP-binding Protein in Cell Mitosis
MA Lian,JIANG Tao,ZHANG Zhong-ming.The Localization of Arabidopsis thaliana Ran Small GTP-binding Protein in Cell Mitosis[J].Journal of Huazhong Agricultural University,2008,27(6).
Authors:MA Lian  JIANG Tao  ZHANG Zhong-ming
Abstract:The expression of Arabidopsis Ran2 mRNA were analysed in different tissues by RT-PCR,and the direct immunofluorescence microscopy was used to determine the subcellular localization of plant Ran proteins during the cell cycle.The results showed that Ran2 is expressed ubiquitously in Arabidopsis roots,stems,leaves and flowers.Ran2 protein was mainly localized at the nuclear envelope during interphase,at the equatorial plate and spindle during anaphase and at the surface of the daughter nuclei during telophase...
Keywords:Arabidopsis Ran2 small GTP-binding protein  anti-Ran2 labelled with FITC  subcellular localization  
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