| 超氧化物歧化酶与苏云金芽胞杆菌高温诱导产黑色素相关 |
| |
| 引用本文： | 刘风侠,杨文君,阮丽芳,孙 明.超氧化物歧化酶与苏云金芽胞杆菌高温诱导产黑色素相关[J].华中农业大学学报,2012,31(1):39-43. |
| |
| 作者姓名： | 刘风侠 杨文君 阮丽芳 孙 明 |
| |
| 作者单位： | 华中农业大学生命科学技术学院/农业微生物学国家重点实验室,武汉,430070 |
| |
| 基金项目： | 国家高技术研究发展计划（“863”计划）（2011AA10A203863）和国家重点基础研究发展计划（973计划）项目（2009CB118902） |
| |
| 摘 要： | 为探讨苏云金芽胞杆菌高温诱导产黑色素机制,利用含mini-Tn10转座子载体pIC333构建苏云金芽胞杆菌油松亚种4CA1,随机插入突变体库,以寻找相关调控基因。通过高温诱导筛选得到1株在42℃产黑色素减弱的菌株;克隆得到mini-Tn10中断基因的部分序列,通过测序及同源性比对分析,确定中断基因为超氧化物歧化酶基因sodA2。
|
| 关 键 词： | 黑色素 转座子 突变体库 超氧化物歧化酶(SOD) 苏云金芽胞杆菌 |
| 收稿时间： | 4/6/2011 12:00:00 AM |
Superoxide dismutase and the melanin production in Bacillus thuringiensis induced by high temperature |
| |
| LIU Feng-xia,YANG Wen-jun,RUAN Li-fang,SUN Ming.Superoxide dismutase and the melanin production in Bacillus thuringiensis induced by high temperature[J].Journal of Huazhong Agricultural University,2012,31(1):39-43. |
| |
| Authors: | LIU Feng-xia YANG Wen-jun RUAN Li-fang SUN Ming |
| |
| Institution: | State Key Laboratory of Agricultural Microbiology/College of Life Science & Technology, Huazhong Agricultural University,Wuhan 430070,China |
| |
| Abstract: | Bacillus thuringiensis subsp.yosoo 4CA1 can not produce melanin at 28℃,while it may possess high yield of melanin when induced at 42℃.In order to study the mechanism of melanin production induced by high temperature,a random insertional mutant library of B.thuringiensis subsp.yosoo 4CA1 was constructed with plasmid pIC333 containing the mini-Tn10 transposon.Compared with the wild-type strain 4CA1,a mutant of producing melanintion weakly at 42℃ was obtained.Then the flanking sequence of mini-Tn10 was cloned and sequenced.The results showed that the gene interrupted by mini-Tn10 was sodA2. |
| |
| Keywords: | melanin transposon mutant library superoxide dismutase(SOD) Bacillus thuringiensis |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《华中农业大学学报》浏览原始摘要信息 |
| 点击此处可从《华中农业大学学报》下载免费的PDF全文 |
