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波尔山羊随机扩增多态DNA标记与体重和体尺的关系
引用本文:曹少先,赵文魁,等.波尔山羊随机扩增多态DNA标记与体重和体尺的关系[J].华中农业大学学报,2001,20(2):159-163.
作者姓名:曹少先  赵文魁
作者单位:1. 南京农业大学动物科技学院,
2. 江苏省徐州市家畜改良站,
基金项目:江苏省科委新的农业技术革命项目! (BG985 0 1-11),江苏省农林厅高产优质肉羊生产综合配套技术研究课题资助项目
摘    要:应用19个波尔山羊个体间多态性较强、重复性好的随机引物对20只5-6月龄波尔山羊公羊组织样DNA进行扩增,共得到156个标记,其中可变标记47个。t检验表明,OPE15.1阳性组腰角宽显著高于阴性组,OPH15.8、OPE15.4阳性组臂端宽显著高于阴性组;而PH13.6、OPH13.8等标记阴性多个体尺、体重性状显著高于阳性组。这说明这瞟标记与特定性状可能存在相关,其遗传基础可能是该标记与控制性状的QTL或主基因连锁。

关 键 词:波尔山羊  RAPD  体重  体尺  育种  遗传标记  生长发育
修稿时间:2000年10月27

Relationship between Random Amplified Polymorphic DNA Markers and Body Weight and Size of Boer Goat
Cao Shaoxian,Yang Liguo,Zhao Wenkui,Zhang Zaobai,Jiang Xunping,\ Mao Dagan.Relationship between Random Amplified Polymorphic DNA Markers and Body Weight and Size of Boer Goat[J].Journal of Huazhong Agricultural University,2001,20(2):159-163.
Authors:Cao Shaoxian  Yang Liguo  Zhao Wenkui  Zhang Zaobai  Jiang Xunping  \ Mao Dagan
Abstract:DNA samples were obtained from tissue of 20 stud Boer goats aged 5~6 months and amplified by 19 selected primers and 156 markers including 47 variable markers were observed in the present study. The results suggested that hip width mean of marker OPE 15.1 positive goat group was significantly higher than that of the negative group, and pin bone width mean of markers OPE 15.4 and OPH 15.8 positive goat group was significantly higher than that of the negative group, while phenotype mean of several traits of markers OPH 13.6 and OPH 13.8 etc negative goat group was significantly higher than that of the positive group . This suggested that these markers and some special traits might be correlative, and the genetic mechanism might be that these markers are linked with QTL or main gene controlled some special traits.
Keywords:Boer goat  RAPD  body weight  body size
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