真菌源壳聚糖酶的纯化及其特征

真菌壳聚糖酶类催化水解壳聚糖,它们在自然界的碳氮循环以及用于处理海洋食品和生产药用寡聚壳聚糖等工业上均有非常重要的意义.自1981年来,已有一些真菌源壳聚糖酶获得了纯化、明确了其特性,并作了序列分析.本文从以下3个方面阐述了真菌壳聚糖酶的近期研究进展1)从Aspergillus fumigatus KH-94, Fusarium solani, Mucor rouxii A*B, Penicillum islandicum, P. spinulosum, Rhodotorula gracilis, Trichoderma reesei, A. oryzae, A. fumigatus Y2K 和 A. fumigatus S-26等真菌中获得的胞内壳聚糖酶的纯化及其特性;2)从T. reesei, A. oryzae, A. fumigatus and Cordyceps sp. MET 1504等真菌中获得的胞外壳聚糖酶的纯化及其特性;3)从T. reesei, Metarhizium anisopliae, A. oryzae and A. fumigatus S-26等真菌中获得的壳聚糖酶氨基酸序列的分析.

Production and Characterization of Fungal Chitosanase

Chitosanases catalyze the hydrolytic degradation of chitosan. They are important for the extensive carbon and nitrogen recycle which occurs in nature and the industrial application which are sea-food-processing and size-specific chitosan oligomer production required particularly in pharmaceutical industries. So far, a number of fungal chitosanases have been purified, characterized and sequenced since 1981. In this paper, the researches of fungal chitosanases are discussed from three aspects : 1. Endo-type chitosanase purification and characterization (Aspergillus fumigatus KH-94, Fusarium solani, Mucor rouxii A. B, Penicillum islandicum , P. spinulosum, Rhodotorula gracilis, Trichoderma reesei, A. oryzae, A. fumigatus Y2K and A. fumigatusS-26). 2. Exo-type fungal chitosanase purification and characterization ( T. reesei, A. oryzae, A. fumigatus and Cordyceps sp. MET 1504). 3. Chitosanase molecular sequence data ( T. reesei, Metarhizium anisopliae , A. oryzae and A. fumigatus S-26). For our present purpose, the studies of fungal chitosanases having very diverse characters are discussed and are compared with our lab chitosanases research results.