| SARS冠状病毒刺突蛋白S144-643的表达及免疫原性分析 |
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| 引用本文： | 费小战,卢海松,郭红燕,谭亚娣,陈焕春,郭爱珍.SARS冠状病毒刺突蛋白S144-643的表达及免疫原性分析[J].华中农业大学学报,2006,25(1):1-5. |
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| 作者姓名： | 费小战 卢海松 郭红燕 谭亚娣 陈焕春 郭爱珍 |
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| 作者单位： | 1. 华中农业大学动物医学院,武汉,430070
2. 华中农业大学动物医学院,武汉,430070;华中农业大学农业微生物学国家重点实验室,武汉,430070 |
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| 摘 要： | 将SARS冠状病毒(SARS-CoV)表面的主要结构蛋白刺突蛋白S144-643的基因片段(nt430-nt1929)克隆入表达质粒pET-28b中,并在大肠杆菌中表达。S144-643蛋白分子质量为55 ku左右,以包涵体形式存在于细菌中。ELISA及Western blot证明,S144-643可以与SARS康复病人的阳性血清发生特异性反应,免疫新西兰大白兔可以诱发产生较高水平的特异性抗体,因而证明原核表达的S144-643具有良好的免疫原性。
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| 关 键 词： | SARS冠状病毒(SARS-CoV) 刺突蛋白(S蛋白) 原核表达 免疫原性 |
| 文章编号： | 1000-2421（2006）01-0001-05 |
| 收稿时间： | 04 25 2005 12:00AM |
| 修稿时间： | 2005年4月25日 |
Expression of SARS-CoV Spike Protein Fragment of S144-643 and Its Immunogenicity |
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| FEI Xiao-zhan,LU Hai-song,GUO Hong-yan,TAN Ya-di,CHEN Huan-chun,GUO Ai-zhen.Expression of SARS-CoV Spike Protein Fragment of S144-643 and Its Immunogenicity[J].Journal of Huazhong Agricultural University,2006,25(1):1-5. |
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| Authors: | FEI Xiao-zhan LU Hai-song GUO Hong-yan TAN Ya-di CHEN Huan-chun GUO Ai-zhen |
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| Institution: | 1. College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070,China; 2. National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070,China |
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| Abstract: | The gene fragment of S144-643 was subcloned into pET-28b and expressed the protein in E.coli.The protein existed mainly in bacterial inclusion bodies.The S144-643 was purified from bacterial inclusion bodies,refolded with the refolding buffer containing reduced glutathione and oxidized glutathione.SDS-PAGE demonstrated the protein with the molecular mass of about 55 ku.The immunogenicity of S144-643 was demonstrated by ELISA and Western blot assay,while the recombinant protein was specifically recognized by the sera from convalescent SARS patients.Furthermore,S144-643 was able to induce high titer of specific antibodies in immunized New Zealand rabbits.The S144-643 protein was expressed in E.coli and was immunologically active. |
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| Keywords: | SARS-associated coronavirus spike(S) glycoprotein prokaryotic expression immunogenicity |
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