| 黄粉虫幼虫免疫血淋巴最佳制样条件的确定 |
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| 引用本文： | 王小平,徐冠军.黄粉虫幼虫免疫血淋巴最佳制样条件的确定[J].华中农业大学学报,1998,17(5):442-446. |
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| 作者姓名： | 王小平 徐冠军 |
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| 作者单位： | 华中农业大学植物保护系 |
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| 摘 要： | 采用正交设计Ｌ２７（３＾１３）的方法对大肠杆菌诱导黄粉幼虫的免疫血淋巴最佳制样条件进行了研究，结果表明：以每虫１０＾５菌量诱导较小的虫体后７２ｈ取样后得到的样品活性最高。
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| 关 键 词： | 黄粉虫 大肠杆菌 免疫血淋巴 制样 抑菌物质 |
DETERMINATION OF OPTIMAL CONDITION OF THE PERPARATION OF IMMUNIZED HEMOLYMPH OF YELLOW MEALWORM ( Tenebrio molitor ) |
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| Wang Xiaoping\ Xu Guanjun.DETERMINATION OF OPTIMAL CONDITION OF THE PERPARATION OF IMMUNIZED HEMOLYMPH OF YELLOW MEALWORM ( Tenebrio molitor )[J].Journal of Huazhong Agricultural University,1998,17(5):442-446. |
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| Authors: | Wang Xiaoping\ Xu Guanjun |
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| Abstract: | Orthogonal experiment has been done to determine the optimal condition in the preparation of the hemolymph of yellow mealworm larvae immunized by Escherichia coli K 12 . The result showed that the best antibacterial activity would be obtained 72 hours after low instar larvae were immunized by injection with 10 5 bacteria per larva. We also discussed the condition when mass sample could be prepared. |
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| Keywords: | yellow mealworm Escherichia coli K 12 orthogonal design antibacterial activity |
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