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贵州苹果砧木资源亲缘关系的ISSR分析 
引用本文:宋莎,吴亚维,季祥彪,宋来庆,文晓鹏.贵州苹果砧木资源亲缘关系的ISSR分析 [J].华中农业大学学报,2013,32(1):19-24.
作者姓名:宋莎  吴亚维  季祥彪  宋来庆  文晓鹏
作者单位:1. 贵州省农业科学院果树科学研究所,贵阳550006;贵州大学贵州省农业生物工程重点实验室,贵阳550025
2. 贵州省农业科学院果树科学研究所,贵阳,550006
3. 贵州大学生命科学学院,贵阳,550025
4. 山东省烟台市农业科学院果树科学研究所,烟台,264000
5. 贵州大学贵州省农业生物工程重点实验室,贵阳,550025
基金项目:贵州省农业科技攻关项目(黔科合字 NY(2006)3003)、贵州省科研机构创新能力建设项目(黔科合院所创能[2010]4009)和贵州省农科院专项(黔农科院院专项[2010]044)
摘    要:利用ISSR标记,对原产于贵州的10份苹果砧木种质及5份外来资源进行亲缘关系分析。结果表明:采用筛选出的10条引物,共扩增出75条重复性好的谱带,其中67条(占89.3%)具有多态性;应用引物827、M05、M08可以有效区分出15份种质资源,构建其DNA的ISSR指纹图谱;聚类分析表明,供试种质间的遗传相似系数在0.49~0.93之间,贵州威宁苹果砧木资源遗传多样性较为丰富,ISSR分析结果与已知苹果砧木间的谱系较为一致。

关 键 词:贵州  苹果砧木  ISSR  指纹图谱  亲缘关系

Genetic analysis of apple rootstock germplasms from Guizhou Province using ISSR markers
SONG Sha , WU Ya-wei , JI Xiang-biao , SONG Lai-qing , WEN Xiao-peng.Genetic analysis of apple rootstock germplasms from Guizhou Province using ISSR markers[J].Journal of Huazhong Agricultural University,2013,32(1):19-24.
Authors:SONG Sha  WU Ya-wei  JI Xiang-biao  SONG Lai-qing  WEN Xiao-peng
Institution:1.Institute of Fruit Tree of Guizhou Academy of Agricultural Sciences, Guizhou Province,Guiyang 550006,China; 2.Key Laboratory of Agricultural Bioengineering at Guizhou Province, Guizhou University,Guiyang 550025,China; 3.College of Life Science,Guizhou University,Guiyang 550025,China; 4.Institute of Fruit Tree in Yantai City Academy of Agricultural Sciences,Yantai 264000,China
Abstract:The genetic relationship of 15 apple rootstock germplasms were studied using ISSR markers.Ten pairs of primers were used and a total of 75 bands were yielded,among which 67 were polymorphic.Primers 827,M05 and M08 could be used for effectively genotyping the 15 germplasms.The systematical cluster analysis showed that 15 apple rootstock germplasms could be divided into three groups: Malus , Pyrus and Sorbus with a genetic similarity coefficient of 0.66,which fitted well with the morphological genealogy.
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