| 气单胞菌和嗜水气单胞菌双重PCR检测方法的建立 |
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| 引用本文： | 潘晓艺,沈锦玉,郝贵杰,姚嘉贇,徐 洋,尹文林,孙逢明,吴颖蕾.气单胞菌和嗜水气单胞菌双重PCR检测方法的建立[J].华中农业大学学报,2011,30(6):759-763. |
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| 作者姓名： | 潘晓艺 沈锦玉 郝贵杰 姚嘉贇 徐 洋 尹文林 孙逢明 吴颖蕾 |
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| 作者单位： | 1. 中国水产科学研究院鱼类健康与免疫重点实验室/浙江省淡水水产研究所,湖州,313001
2. 浙江省富阳市水产技术服务站,杭州,311400 |
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| 基金项目： | 公益性行业(农业)科研专项经费(200803013); 浙江省科技计划项目(2008C22058); 湖州市科研计划项目(2007YN22) |
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| 摘 要： | 针对GenBank中登录的气单胞菌属(Aeromonas)的毒力基因甘油磷脂胆固醇酰基转移酶基因(GCA T)和嗜水气单胞菌(Aeromonash ydrophila)的16S rRNA基因的保守区设计2对特异性引物.通过进行双重PCR反应体系优化,PCR产物的测序鉴定和特异性试验,建立了一种能同时检测气单胞菌(Aer...
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| 关 键 词： | 气单胞菌 嗜水气单胞菌 16S rRNA GCAT基因 双重PCR |
| 收稿时间： | 2010/12/2 0:00:00 |
Development of a duplex PCR for detection of Aeromonas spp. and Aeromonas hydrophila |
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| PAN Xiao-yi,SHEN Jin-yu,HAO Gui-jie,YAO Jia-yun,XU Yang,YIN Wen-lin,SUN Feng-ming,WU Ying-lei.Development of a duplex PCR for detection of Aeromonas spp. and Aeromonas hydrophila[J].Journal of Huazhong Agricultural University,2011,30(6):759-763. |
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| Authors: | PAN Xiao-yi SHEN Jin-yu HAO Gui-jie YAO Jia-yun XU Yang YIN Wen-lin SUN Feng-ming WU Ying-lei |
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| Institution: | PAN Xiao-yi1 SHEN Jin-yu1 HAO Gui-jie1 YAO Jia-yun1 XU Yang1 YIN Wen-lin1 SUN Feng-ming2 WU Ying-lei1 1.Key Laboratory of Fish Health and Immunology,Chinese Academy of Fishery Science/ Zhejiang Institute of Freshwater Fisheries,Huzhou 313001,China,2.Fuyang Fishery Technical Extension Station of Zhejiang Province,Hangzhou 311400 |
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| Abstract: | Aeromonas is one of the major pathogens which frequently cause the bacterial diseases in freshwater fish terrapin and crustaceans.Aeromonas hydrophila caused economic losses widely.In order to detect Aeromonas spp.and Aeromonas hydrophila,two pairs of specific primers were designed according to the glycerophospholipid-cholesterol acyltransferase(GCAT) gene of Aeromonas spp.and the 16S rRNA gene of Aeromonas hydrophila.So a duplex PCR assay was developed to amplify the 16S rRNA gene and the GCAT gene.The rea... |
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| Keywords: | Aeromonas spp Aeromonas hydrophila 16S rRNA GCAT gene duplex PCR |
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