黄颡鱼外周血细胞的组成及其显微与超显微结构

将土池饲养的黄颡鱼(Pelteobagrus fulvidraco)成鱼在室内养鱼系统中充分恢复,采用光镜和电镜技术对外周血细胞的组成及结构进行观察。在血涂片上可鉴别出红细胞、血栓细胞、淋巴细胞、嗜中粒细胞和单核细胞,电镜观察可确认这些细胞类型。血涂片上还观察到幼红细胞及正在分裂的红细胞。血栓细胞是数量仅次于红细胞的血细胞,在白细胞分类计数中占78%。淋巴细胞是核质比最高的血细胞,在白细胞分类计数中占17.5%。一种与斑点叉尾鮰非特异性细胞毒性细胞相似的淋巴细胞被观察到。嗜中粒细胞是所观察到的唯一一种有粒细胞,是最大的白细胞,在白细胞分类计数中占3.7%。在嗜中粒细胞中只观察到一种有丝状内涵物的特殊颗粒,呈椭圆状或杆状。单核细胞在白细胞分类计数中不超过1%。

Light and Electron Microscopic Observations on the Peripheral Blood Cells of Yellow Catfish, Pelteobagrus fulvidraco (Bagridae: Teleostei)

Earth-pond-reared adult yellow catfishwere fully recovered in the indoor laboratory recycle system and usedto examine the normal haematological features of yellow catfish(Pelteobagrus fulvidraco) by both light and transmission electron microscope(TEM).Erythrocytes,thrombocytes,lymphocytes,neutrophilsand monocytes were identified in blood smears and confirmed by TEM.Immature erythrocytes and occasionally dividing erythrocytes were also present in smears.The thrombocyteswere the most abundant cell type after erythrocytes,representing 78% in differential leucocyte counts(DLC).The lymphocytes constituted 17.5% in DLC and had the highest nuclear:cytoplasmic ratio of all the leucocytes observed.A unique lymphocyte that was morphologically similar to the channel catfish non-specific cytotoxic cells(NCCs)was observed.Neutrophils,the only type of granulocytesidentifiedin yellow catfish,had the largest mean cell diameter of all leucocytes and constituted 3.7% in DLC.The only type of the specific granules in the neutrophils contained fibrillous material and were ovoid or rod-like in shape.Monocytes represented less than 1% in DLC.