拮抗细菌的筛选及其对油菜菌核病的防治效果

SCREENING BACTERIAL ANTAGONISTS FOR USE AS THE BIOCONTROL AGENTS FOR THE SCLEROTINIA DISEASE (SCLEROTINIA SCLEROTIORUM) OF RAPESEED

Bacterial isolates were obtained by isolation from the decayed sclerotia of sclerotinia sclerotiorum and the surface of the above ground tissul of seven sepcies of plants.Dual cultures on PDA with 0.3% beef extract showed that, of the total of 163 bacterial isolats obtained, 31 were antagonistic to Scleroiinia sclerotiorum.Hyphal tips of S.sclerotiorum at the inhibition zone turned brown and their morphological alternations were microscopicilly obervde, including granulation of the cytoplasma,leakage of the granulated cytoplasma, necrosis of the hyphal wall and mass production of microconidia. When screened through in vitro tests on the rapeseed leaf disks, greenhouse pot experiment on the rapeseed seedlings and a preliminary field test on the flowering rapeseed plants, three bacterial antagonists were effective at suppressing the sclerotinia disease of rapeseed.