| 矮牵牛的快速繁殖 |
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| 引用本文： | 邹继美.矮牵牛的快速繁殖[J].吉林农业科技学院学报,2008,17(4):18-21. |
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| 作者姓名： | 邹继美 |
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| 作者单位： | 吉林农业大学,长春130118 |
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| 摘 要： | 矮牵牛带芽嫩茎、嫩叶作为外植体，经培养都能再生完整植株。诱导分化阶段，带芽嫩茎以腋芽发育为主，也有不定芽发育，最适培养基MS＋6-BA0．5mg／L＋NAA0．1mg／L或MS＋6-BA1．0mg／L＋NAA0．5mg／L；叶片以不定芽发育为主，在MS＋6-BA0．5mg／L＋NAA0．5mg／L及MS＋6-BA1．0mg／L＋NAA0．1mg／L培养基上，丛生芽分化效果最好。继代增殖阶段，切割新梢接种于MS培养基或切割丛生芽接种于MS＋6-BA0．5mg／L培养基，均可获得6～10倍的增殖。生根培养阶段，1／2MS＋IBA0．01—0．05mg／L培养基的生根效果好，移栽成活率可达95．6％。
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| 关 键 词： | 矮牵牛 离体再生 快速繁殖 |
Rapid Propagation of Petunia hybrida |
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| ZOU Jimei.Rapid Propagation of Petunia hybrida[J].Journal Of Jilin Agricultural Science And Technology College,2008,17(4):18-21. |
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| Authors: | ZOU Jimei |
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| Institution: | ZOU Jimei (Jilin Agricultural University,Changchun 130118,China) |
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| Abstract: | The stems with a bud and the leaves of Petunia hybrida as explants were cultured in tubes and plantlet regeneration was all accomplished.In induction and differentiation stage: The stems with a bud produced axillary buds first,and adventitious buds too.The most appropriate media were MS implementation of 6-BA0.5mg/L and NAA0.1mg/L,or 6-BA1.0mg/L and NAA0.5mg/L;The leaves differentiated adventitious buds.On MS with 6-BA0.5mg/L and NAA0.5mg/L,or on MS with 6-BA1.0mg/L and NAA0.1mg/L,rosette buds grew well.New... |
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| Keywords: | Petunia hybrida plantlet regeneration rapid propagation |
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