| 仙人掌超氧化物歧化酶提取与纯化研究 |
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| 引用本文： | 刘晓丹,王波.仙人掌超氧化物歧化酶提取与纯化研究[J].吉林农业科技学院学报,2010(2):8-10,124. |
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| 作者姓名： | 刘晓丹 王波 |
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| 作者单位： | 吉林农业科技学院生物工程学院,吉林132101 |
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| 摘 要： | 以仙人掌为试验材料,采用硫酸铵分级沉淀法得到超氧化物歧化酶（Superoxide Dismutase,SOD）粗酶液。然后用Sephadex（葡聚糖凝胶）G-75层析柱层析对粗酶液进行进一步纯化,最后用邻苯三酚法测定酶活力,并对酶活力主要影响因素（包括pH值、温度、抑制剂）进行了研究。结果显示仙人掌SOD酶比活力为751.56U.mg-1。pH值为7.5~8.5时酶活力最大,稳定性也好。温度维持在25~30℃时酶活力最高。由于过氧化氢和氰化钾均能使仙人掌SOD的酶活力显著下降,因而可以初步判断从仙人掌中提取到的SOD是Cu.Zn-SOD。
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| 关 键 词： | 仙人掌 SOD 分离 纯化 酶活力 测定 |
Purification and Identification of Superoxide Dismutase from Cactus |
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| LIU Xiaodan,WANG Bo.Purification and Identification of Superoxide Dismutase from Cactus[J].Journal Of Jilin Agricultural Science And Technology College,2010(2):8-10,124. |
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| Authors: | LIU Xiaodan WANG Bo |
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| Institution: | LIU Xiaodan,WANG Bo(Jilin Agricultural Science and Technology College College of Bioengineering,Jilin 132101,China) |
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| Abstract: | Introduced the isolation and purification processes of SOD from cactus.First,obtained rough enzyme by ammonium sulfate fractional precipitation,then make further purification by chromatography using Sephadex G-75.Last,the activity of SOD was measured with pyrogallol,and influencing factor of SOD activity,including temperature,pH and inhibitors was studied in the experiment.The specific activity obtained was 751.56U/mg.The results showed that when pH was between 7.5 to 8.5 and the temperature was between 25 ... |
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| Keywords: | cactus SOD isolation purification activity determination |
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