| 甘蓝型油菜黄籽基因RAPD标记的初步研究 |
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| 引用本文： | 董艳珍,李宇,胥婷,李关荣,李加纳.甘蓝型油菜黄籽基因RAPD标记的初步研究[J].西南农业大学学报,2004,26(5):529-531. |
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| 作者姓名： | 董艳珍 李宇 胥婷 李关荣 李加纳 |
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| 作者单位： | [1]西南农业大学农学与生命科学学院,重庆400110 [2]内江师范学院生物系,四川内江641112 |
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| 基金项目： | 国家863项目(2001AA241103),(2002AA2070097),重庆"十五"种子创新工程资助项目 |
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| 摘 要： | 采用RAPD技术,以甘蓝型油菜近等基因系03L1(黑籽)和03L2(黄籽)为材料,对200个10nt的RAPD引物进行了筛选。有18个引物能在近等基因系间扩增出差异片段,其中6个引物在重复实验中表现出很好的重复性。这6个引物扩增出的6个特异片段可能是甘蓝型油菜黄籽基因的RAPD标记。
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| 关 键 词： | 甘蓝型油菜 黄籽基因 近等基因系 RAPD标记 |
| 文章编号： | 1000-2642(2004)05-0529-03 |
RAPD MARKERS FOR YELLOW SEED GENE IN BRASSICA NAPUS |
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| DONG Yan-zhen,LI Yu,XU Ting,LI Guan-rong,LI Jia-na.RAPD MARKERS FOR YELLOW SEED GENE IN BRASSICA NAPUS[J].Journal of Southwest Agricultural University,2004,26(5):529-531. |
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| Authors: | DONG Yan-zhen LI Yu XU Ting LI Guan-rong LI Jia-na |
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| Institution: | DONG Yan-zhen~1,LI Yu~2,XU Ting~1,LI Guan-rong~1,LI Jia-na~1 |
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| Abstract: | Two near-isogenic lines of Brassica napus, 03L1 (black seeded) and 03L2 (yellow-seeded), were analyzed by RAPD technique and 200 RAPD primers were screened. Eighteen primers showed polymorphism between the two lines. In replicated experiments, 6 of the 18 primers showed good stability and repeatability. The 6 specific fragments might be RAPD markers for yellow seed coat color in B. napus. |
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| Keywords: | Brassica napus yellow seed color gene near-isogenic line RAPD marker |
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