| 黄瓜组织培养研究 |
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| 引用本文： | 梅茜,张兴国.黄瓜组织培养研究[J].西南农业大学学报,2002,24(3):266-267. |
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| 作者姓名： | 梅茜 张兴国 |
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| 作者单位： | 西南农业大学园艺系 重庆400716
(梅茜)，西南农业大学园艺系 重庆400716(张兴国) |
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| 摘 要： | 对黄瓜组织培养的条件作了探讨。结果表明，苗龄1－2d的子叶，在附加1.0mg/L BA,2.0mg/L AgNO3和1.0mg/L ABAr MS的固体培养基上，最适于不定芽分化，1个月内的再生率达50％左右，其中苗龄和ABA的影响最为显著，加入适量的AgNO3可改善黄瓜愈伤组织的质地、促进芽的形成。
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| 关 键 词： | 黄瓜 组织培养 不定芽分化 固体培养基 再生率 苗龄 ABA 愈伤组织 |
| 文章编号： | 1000-2642(2002)03-0266-02 |
| 修稿时间： | 2001年11月15 |
STUDIES ON TISSUE CULTURE OF CUCUMBER |
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| MEI Qian,ZHANG Xing-guo.STUDIES ON TISSUE CULTURE OF CUCUMBER[J].Journal of Southwest Agricultural University,2002,24(3):266-267. |
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| Authors: | MEI Qian ZHANG Xing-guo |
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| Institution: | MEI Qian,ZHANG Xing-guo * |
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| Abstract: | Best results of adventitious bud differentiation were obtained when 1- or 2-day-old cotyledons were cultured on MS medium supplemented with BA 1.0 mg/L, AgNO 3 2.0 mg/L, ABA 1.0 mg/L, 3% sucrose and 0.6% agar, and a regeneration rate of 50% was achieved within one month. The age of the seedlings and ABA showed the greatest effects. The addition of AgNO 3 to the medium improved the quality of the calli and helped the formation of regenerated buds. |
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| Keywords: | cucumber tissue culture adventitious bud differentiation |
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