| 蛋白酶处理对雪茄芯叶品质的影响 |
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| 引用本文： | 寇明钰,汪长国,戴亚,曾代龙,冯广林,张燕,刘林,杨文敏,李宁,吴艳,赵敏,贾玉红,雷金山.蛋白酶处理对雪茄芯叶品质的影响[J].西南农业大学学报,2011,33(10). |
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| 作者姓名： | 寇明钰 汪长国 戴亚 曾代龙 冯广林 张燕 刘林 杨文敏 李宁 吴艳 赵敏 贾玉红 雷金山 |
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| 作者单位： | 卷烟减害降焦四川省重点实验室;川渝中烟工业公司技术研发中心;云南省烟草质量监督检测站; |
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| 基金项目： | 国家高新技术研究发展计划(863计划)(2010AA101506-6); 四川省立项项目(2009JY0156); 川渝中烟博士后基金项目(2010-01)资助 |
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| 摘 要： | 以雪茄芯叶为材料,在单一蛋白酶处理的基础上,采用正交试验进行组合蛋白酶处理,研究蛋白酶处理对雪茄芯叶蛋白质含量和芯叶品质的影响.结果表明:单一蛋白酶处理时,中性蛋白酶和碱性蛋白酶效果较好,芯叶蛋白质含量分别降低18.98%和17.54%;组合蛋白酶处理时,最佳发酵条件为雪茄芯叶用组合蛋白酶(中性蛋白酶120U/g烟叶+碱性蛋白酶4×10-4 AU/g烟叶)于45℃、85%湿度下处理8h.经组合蛋白酶处理后,芯叶蛋白质含量降低25.38%,化学成分更加协调,感官品质得到提高.
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| 关 键 词： | 雪茄芯叶 蛋白酶 正交试验 |
Effect of Protease Treatment on Inner Quality of Cigar Filler Tobacco |
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| KOU Ming-yu,WANG Chang-guo,DAI Ya,ZENG Dai-long,FENG Guang-lin,ZHANG Yan,LIU Lin,YANG Wen-min,LI Ning,WU Yan,ZHAO Min,JIA Yu-hong,LEI Jin-shan, .Sichuan Key Laboratory for Harmful Component , Tar Reduction in Cigarette,Chengdu ,China,.Technical Research Center,Chuanyu Branch of China Tobacco Corporation,.Yunnan Provincial Tobacco Quality Supervision , Inspection Station,Kunming.Effect of Protease Treatment on Inner Quality of Cigar Filler Tobacco[J].Journal of Southwest Agricultural University,2011,33(10). |
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| Authors: | KOU Ming-yu WANG Chang-guo DAI Ya ZENG Dai-long FENG Guang-lin ZHANG Yan LIU Lin YANG Wen-min LI Ning WU Yan ZHAO Min JIA Yu-hong LEI Jin-shan Sichuan Key Laboratory for Harmful Component Tar Reduction in Cigarette Chengdu China Technical Research Center Chuanyu Branch of China Tobacco Corporation Yunnan Provincial Tobacco Quality Supervision Inspection Station Kunming |
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| Institution: | KOU Ming-yu1,2,WANG Chang-guo1,DAI Ya1,ZENG Dai-long1,FENG Guang-lin1,ZHANG Yan3,LIU Lin1,YANG Wen-min1,LI Ning1,WU Yan1,ZHAO Min1,JIA Yu-hong1,LEI Jin-shan1,2 1.Sichuan Key Laboratory for Harmful Component and Tar Reduction in Cigarette,Chengdu 610066,China,2.Technical Research Center,Chuanyu Branch of China Tobacco Corporation,3.Yunnan Provincial Tobacco Quality Supervision and Inspection Station,Kunming 650106 |
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| Abstract: | On the foundation of single protease treatments of cigar filler tobacco,an orthogonal experiment was conducted,in which the effects of mixed-protease treatment and other three factors on protein content of cigar filler tobacco and its inner quality were studied.The results indicated that of the single protease treatments,neutral protease and alkaline protease had better effects,with a protein degradation percentage of 18.98% and 17.54%,respectively,and that in the orthogonal experiment,the optimum parameter... |
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| Keywords: | cigar filler tobacco protease orthogonal experiment |
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