外源亚精胺对盐胁迫下颠茄生理特性和生物碱积累的影响

以颠茄为试验材料,在盐胁迫下对叶片喷施不同浓度的亚精胺(Spd)溶液,研究Spd对颠茄相关生理特性和叶片干物质中莨菪碱和东莨菪碱质量分数的影响.试验结果表明:喷施较低浓度(0.1mmol/L,0.3mmol/L)的Spd溶液,可使颠茄的抗盐能力增强,叶绿素质量分数上升,丙二醛(MDA)、可溶性糖、可溶性蛋白(SP)、脯氨酸(Pro)质量分数降低,过氧化物酶(POD)、超氧化物歧化酶(SOD)活性均降低,莨菪碱质量分数上升,并且经0.1mmol/L的Spd溶液处理的颠茄叶片东莨菪碱质量分数是对照组的3.44倍.因此,叶片喷施适宜浓度的Spd溶液能够有效缓解盐胁迫对颠茄的伤害,使植株恢复正常生长,提高颠茄莨菪碱和东莨菪碱的质量分数,这表明亚精胺能够增强颠茄的抗盐能力,提高其有效药用成分产量.

Effects of Exogenous Spermidine on Physiological Characteristics and Tropane Alkaloids Accumulation of Atropa belladonna Under Salt Stress

In order to find a method for improving the salt-resistant ability and the content of tropane alkaloids of Atropa belladonna under salt stress, different concentration of Spermidine(Spd) were sprayed on their leaves to study the effects on physiological characteristics and tropane alkaloids accumulation. Compared with the control group, lower concentration Spd increased the content of chlorophyll, while decreased the content of malondialdehyde(MDA), soluble sugar, soluble protein(SP), proline(Pro). Moreover, the antioxidant enzyme(POD, SOD)activity was lower than the group under salt stress. In respect of tropane alkaloids accumulation, Spd led to more production of hyoscyamine, and scopolamine has increased to 3.44 fold than the control group at the concentrate of 0.1 mmol/L. However, high concentration Spd cannot have this effect. Thus, proper concentration of Spd is conductive to the growth and production of chemical ingredients of Atropa belladonna when suffered salt stress.