| BLM642-1290重组解旋酶的优化表达 |
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| 引用本文： | 陈祥,骆衡,段丽霞,张勇,许厚强.BLM642-1290重组解旋酶的优化表达[J].西南农业大学学报,2011,33(2). |
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| 作者姓名： | 陈祥 骆衡 段丽霞 张勇 许厚强 |
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| 作者单位： | 贵州省动物遗传育种与繁殖重点实验室贵州大学动物科学学院;贵州大学教育部绿色农药与农业生物工程重点实验室; |
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| 基金项目： | 国家自然科学基金资助项目(30660043); 教育部博士点基金资助项目(200806570003); 贵州省优秀人才省长基金资助项目(200822); 贵州省创新科研基金资助项目(SYN2009005) |
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| 摘 要： | Bloom综合症(Bloom s syndrome)是人类一种少见的常染色体隐性遗传疾病,BLM基因突变导致这种疾病的发生,患者染色体极不稳定,是多种癌症的易患体,其致病机理不清楚.该研究在优化诱导表达温度、时间、IPTG质量浓度、pH值、培养基种类以及培养基成分的基础上,建立了BLM642-1290重组蛋白表达、分离和纯化方法.最优表达条件为IPTG0.45 mmol/L,诱导温度18℃,诱导表达时间20 h,pH值7.0.在LB培养基中添加终质量浓度为5 mmol/L的EDTA能够增加蛋白的表达量.该实验所建立的方法为进一步开展Bloom综合症的研究奠定了基础.
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| 关 键 词： | BLM642-1290 优化 表达 |
Optimized Expression of BLM642-1290 Recombinant Helicase |
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| CHEN Xiang,LUO Heng,DUAN Li-xia,ZHANG Yong,XU Hou-qiang.Guizhou Key Laboratory of Animal Genetics,Breeding , Reproduction,College of Animal Science,Guizhou University,Guiyang ,China,.Ministry of Education Key Laboratory of Green Pesticide , Ago-Bioengineering.Optimized Expression of BLM642-1290 Recombinant Helicase[J].Journal of Southwest Agricultural University,2011,33(2). |
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| Authors: | CHEN Xiang LUO Heng DUAN Li-xia ZHANG Yong XU Hou-qiangGuizhou Key Laboratory of Animal Genetics Breeding Reproduction College of Animal Science Guizhou University Guiyang China Ministry of Education Key Laboratory of Green Pesticide Ago-Bioengineering |
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| Institution: | CHEN Xiang1,2,LUO Heng1,DUAN Li-xia1,ZHANG Yong1,XU Hou-qiang11.Guizhou Key Laboratory of Animal Genetics,Breeding and Reproduction,College of Animal Science,Guizhou University,Guiyang 550025,China,2.Ministry of Education Key Laboratory of Green Pesticide and Ago-Bioengineering |
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| Abstract: | Bloom's syndrome(BS) is an autosomal recessive disorder characterized by genomic instability and a predisposition to many of cancers.Mutations of the BLM gene(encoding a BLM helicase) leads to the disease,but its pathologic mechanism remains poorly understood.In this study,an effective approach was established for induced expression,separation and purification of BLM642-1290 recombinant protein,based on the optimization of induced expression temperature,time,IPTG concentration,pH,variety of medium and mediu... |
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| Keywords: | BLM642-1290 optimization expression |
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