| 羟基喜树碱(HCPT)对乳腺癌细胞中TIMP-3表达及细胞侵袭能力的影响 |
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| 引用本文： | 周萍,汪洋,付利娟.羟基喜树碱(HCPT)对乳腺癌细胞中TIMP-3表达及细胞侵袭能力的影响[J].西南农业大学学报,2011,33(4). |
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| 作者姓名： | 周萍 汪洋 付利娟 |
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| 作者单位： | 重庆医科大学中医药学院;重庆医科大学公共卫生学院; |
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| 基金项目： | 重庆市科委自然科学基金资助项目(CSCT2009BB52712008BB7394) |
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| 摘 要： | 目的:探索羟基喜树碱对乳腺癌细胞MCF-7细胞信号转导相关基因金属蛋白酶组织抑制剂-3(TIMP-3)表达的影响,寻找羟基喜树碱(HCPT)在乳腺癌治疗中的潜在新靶点.方法:分别采用Q-PCR,Western-blot和免疫细胞化学等方法验证不同剂量组HCPT诱导MCF-7细胞的TIMP-3表达特征.结果:不同质量浓度的HCPT均可在mRNA水平和蛋白水平诱导MCF-7细胞的TIMP-3表达上调,且具有统计学意义,与前期的基因芯片研究结果相符合;实验同时观察了HCPT对细胞侵袭迁移能力的影响,结果亦显示HCPT可在TIMP-3表达上调的同时抑制细胞侵袭迁移能力.结论:HCPT可诱导乳腺癌细胞的TIMP-3上调表达,抑制细胞侵袭能力.
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| 关 键 词： | 羟基喜树碱(HCPT) 乳腺癌 金属蛋白酶组织抑制剂-3(TIMP-3) |
The Effects of Hydroxycamptothecin(HCPT)on the Expression of TIMP-3 and Cell Invasion of Breast Tumor Cells |
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| ZHOU Ping,WANG Yang,FU Li-juan.School of Troditional Medicine,Chongqing Medical University,Chongqing ,China,.School of Public Health.The Effects of Hydroxycamptothecin(HCPT)on the Expression of TIMP-3 and Cell Invasion of Breast Tumor Cells[J].Journal of Southwest Agricultural University,2011,33(4). |
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| Authors: | ZHOU Ping WANG Yang FU Li-juanSchool of Troditional Medicine Chongqing Medical University Chongqing China School of Public Health |
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| Institution: | ZHOU Ping1,WANG Yang2,FU Li-juan11.School of Troditional Medicine,Chongqing Medical University,Chongqing 400016,China,2.School of Public Health |
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| Abstract: | Objective: In this paper,the effects of HCPT(10-hydroxy-camptothecin) on the expression of TIMP-3 in breast tumor cell line MCF-7 was observed to seek new potential target points of HCPT for the treatment of breast tumor.Methods: Q-PCR,Western-blot and cytoimmochemical method were employed to re-testify the expression of TIMP-3,an up-regulated expressed gene,in a previous microarray result.Result: HCPT at different concentrations induced up-regulated expression of TIMP-3 in the MCF-7 cell line(p<0.05),a res... |
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| Keywords: | HCPT breast tumor TIMP-3 |
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