| 正交试验法在牛蒡组织培养中的应用 |
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| 引用本文： | 何建,梁国鲁.正交试验法在牛蒡组织培养中的应用[J].西南农业大学学报,2004,26(6):696-698,702. |
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| 作者姓名： | 何建 梁国鲁 |
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| 作者单位： | 西南农业大学园艺园林学院，西南农业大学园艺园林学院 重庆 400716，重庆 400716 |
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| 摘 要： | 应用正交设计法研究BA，NAA及蔗糖3个因子对牛蒡(Arctium lappa L．)组培苗的增殖率的影响，同时考察了NAA，BA及不同浓度的Ms培养基3个因子对牛蒡组培苗生根培养的影响，各试验均按正交表L9(3^4)形成3因素、3水平的不同配比。试验结果表明：最佳的增殖培养基是MS BA2．0mg／L NAA0．05mg／L 蔗糖30g／L，最适于生根的培养基是1／2MS BA0．1mg／L NAA0．05mg／L 30g／L蔗糖。
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| 关 键 词： | NAA 牛蒡 BA 组培苗 组织培养 蔗糖 Ms培养基 MS 正交试验法 影响 |
| 文章编号： | 1000-2642(2004)06-0696-03 |
APPLICATION OF ORTHOGONAL EXPERIMENT IN TISSUE CULTURE OF ARCTIUM LAPPA L. |
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| HE Jian,LIANG Guo-lu.APPLICATION OF ORTHOGONAL EXPERIMENT IN TISSUE CULTURE OF ARCTIUM LAPPA L.[J].Journal of Southwest Agricultural University,2004,26(6):696-698,702. |
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| Authors: | HE Jian LIANG Guo-lu |
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| Abstract: | In order to analyze the effect of different contents of hormones and sucrose in the medium on tufted-bud induction and tufted-bud rooting of Arctium lappa L., tissue culture of the plant with the seeds as the explants was carried out, in which the orthogonal design, L_9(3~4), was used. The results indicated that MS supplemented with BA 2.0 mg/L NAA 0.05 mg/L sucrose 30 g/L was suitable for its multiplication and1/2MS supplemented with BA 0.1 mg/L NAA 0.05 mg/L sucrose 30 g/L was optimal for rooting. |
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| Keywords: | Arctium lappa L tissue culture orthogonal experiment |
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