| 甘蓝型油菜与羽衣甘蓝远缘杂交初步研究 |
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| 引用本文： | 陈树忠,
殷家明,
唐章林,
李加纳,.甘蓝型油菜与羽衣甘蓝远缘杂交初步研究[J].西南农业大学学报,2000,22(3):208-210. |
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| 作者姓名： | 陈树忠 殷家明 唐章林 李加纳 |
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| 作者单位： | 西南农业大学农学系,重庆 |
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| 基金项目： | 国家“九五”育种攻关资助项目!(96 - 0 0 2 - 0 2 - 13 - 0 1) |
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| 摘 要： | 对甘蓝型油菜和羽衣甘蓝种间杂交杂种进行胚胎挽救研究，实验结果表明，采用Ｂ５＋１．０ｍｇ／ＬＢＡ＋０．２ｍｇ／ＬＮＡＡ＋０．５％Ｃａｒｂｏｎ＋３０ｇ／ＬＳｕｇａｒ＋８．０ｇ／ＬＡｇａｒ培养基对甘蓝型油菜和羽衣甘蓝杂交子房培养效果较为理想，通过对取材时间研究发现，取授粉后１０天子房培养获得种子成功率较高。实验共获得了７粒种子，其中，３粒萌发得到再生植株，细胞学和酯酶同工麦鉴定和结果表明都为真杂种。
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| 关 键 词： | 甘蓝型油菜 羽衣甘蓝 远缘杂交 杂种鉴定 黄籽 |
A PRELIMINARY STUDY ON INTERSPECIFIC HYBRIDIZATION BETWEEN BRASSICA NAPUS AND B. OLERACEA VAR. ACEAPHALA |
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| CHEN Shu-zhong,YIN Jia-ming,TANG Zhang-lin,LI Jia-na.A PRELIMINARY STUDY ON INTERSPECIFIC HYBRIDIZATION BETWEEN BRASSICA NAPUS AND B. OLERACEA VAR. ACEAPHALA[J].Journal of Southwest Agricultural University,2000,22(3):208-210. |
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| Authors: | CHEN Shu-zhong YIN Jia-ming TANG Zhang-lin LI Jia-na |
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| Institution: | CHEN Shu-zhong,YIN Jia-ming,TANG Zhang-lin,LI Jia-na(Department of Agronomy,Southwest Agricultural University,Chongqing,China |
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| Abstract: | The embryo rescue technique was applied to obtain interspecific hybrids between Brassica napus and B.oleracea var. aceaphala. Of the media studied, B 5 supplemented with 1.0 mg/L BA 0.2 mg/L NAA 0.5% carbon 30 g/L sugar 8.0 g/L agar gave the most satisfactory results for ovary culture. Ovaries taken 4,7,10,13, and 16 days after pollination were used for culture, and those taken 10 days after pollination appeared most efficient for seed production. Altogether 7 seeds were obtained in the experiment and three of them germinated on MS medium and grew into plantlets. Cytological study and esterase isozyme verification showed that they were all true hybrids between B. napus and B.oleracea var. aceaphala. |
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| Keywords: | Brassica napus Brassica oleracea var aceaphala distant hybridization |
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