早孕小鼠子宫内膜ICAM-1的表达及意义

探讨细胞间粘附分子-1（ICAM-1）基因及蛋白在早孕小鼠子宫内膜的表达及其意义方法：收集妊娠1-5d的早孕小鼠子宫内膜，利用RT-PCR、免疫组织化和Western-blot方法检测ICAM-1基因及蛋白的表达及分布；着床期小鼠单侧宫角注射ICAM-1单抗，观察胚胎着床变化．结果：1．ICAM-1 mRNA孕D1到D3逐渐增高，D4达最高，D5开始降低；2．子宫内膜上皮细胞，基质细胞，血管上皮细胞均有ICAM-1的表达，阳性细胞数量、表达强度和表达时间呈现一定规律性；3．ICAM-1D1、D2、D3略有升高，D4有强表达，D5略有下降；4．单侧宫角注射ICAM-1单抗后，该侧胚胎发生流产．结论：ICAM-1在早孕小鼠子宫内膜的表达变化及分布，提示ICAM-1参与了胚泡着床，ICAM-1可能起调节子宫内膜的局部免疫作用．

Expression of ICAM-1 on Endometrium of Early Pregnant Mice and its Functions

Endometrium samples were collected from Day 1 to Day 5 in early pregnant mice, and the expression level of ICAM-1 (intercellular adhesion molecule-1) mRNA on it was determined by RT-PCR and the expression level of ICMA-1 were determined by immunohisto chemistry and Western blotting. Monoclone antibody of ICAM-1 was injected into the uterus on one side of the early pregnant mice to observe the changes of the embryo. The expression level of ICAM-1 mRNA on the endometrium increased steadily to Day 4 and declined gradually thereafter. Expression of ICMA-1 was detected on endometrium epithelia, matrix cells and blood vessel epithelia and the quantity of its positive cells and the intensity and duration of the expression showed a definite pattern. In Western blotting assay, ICMA-1 slightly increased from D1 to D 3 and showed a strong expression on Day 4, which was followed by a decline thereafter. The embryo aborted spontaneously on the side of the injection of monoclone antibody of ICAM-1. These results suggest that ICAM-1 may be involved in the process of blastosyst implantation and regulate the local immunity of the endometrium.