| 羊源杀鲑气单胞菌16S rRNA基因的克隆测序及分析 |
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| 引用本文： | 林如涛,周作勇,王小林,聂奎.羊源杀鲑气单胞菌16S rRNA基因的克隆测序及分析[J].西南农业大学学报,2011,33(8). |
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| 作者姓名： | 林如涛 周作勇 王小林 聂奎 |
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| 作者单位： | 西南大学荣昌校区动物医学系;西南大学动物科技学院; |
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| 基金项目： | 中央高校基本科研业务费专项基金资助项目(XDJK2010C099) |
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| 摘 要： | 从重庆市丰都县无菌采集山羊血液,提取全血基因组,用16SrRNA基因的引物进行PCR扩增,得到2条长约1 500bp的扩增片段,将其克隆到pMD19-T载体后进行测序,并与3条豚鼠气单胞菌,3条嗜水气单胞菌,3条中间气单胞菌,4条杀鲑气单胞菌,2条温和气单胞菌,3条维氏气单胞菌和5条舒氏气单胞菌16SrRNA基因序列进行系统发育分析.结果表明:所克隆的基因片段长度均为1 507bp,GenBank登录号为JF823571和JF823572.系统发育分析发现2条序列均被聚类到杀鲑气单胞菌群,并与温和气单胞菌聚类到一个大的分支.同源性比对结果显示所获得的序列与杀鲑气单胞菌法国株(ATCC33658T)X74681和阿根廷株AF134065的同源性最高,均为99.6%,表明重庆地区存在受杀鲑气单胞菌感染的山羊.
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| 关 键 词： | 山羊 杀鲑气单胞菌 16S rRNA 克隆测序 |
Cloning and Sequence Analysis of the 16S rRNA Gene of Aeromonas salmonicida in Goat |
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| LIN Ru-tao,ZHOU Zuo-yong,WANG Xiao-lin,NIE Kui.Cloning and Sequence Analysis of the 16S rRNA Gene of Aeromonas salmonicida in Goat[J].Journal of Southwest Agricultural University,2011,33(8). |
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| Authors: | LIN Ru-tao ZHOU Zuo-yong WANG Xiao-lin NIE Kui |
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| Institution: | LIN Ru-tao1,ZHOU Zuo-yong1,WANG Xiao-lin1,NIE Kui2 1.Department of Veterinary Medicine,Southwest University(Rongchang Campus),Rongchang Chongqing 402460,China,2.School of Animal Science and Technology,Southwest University,Chongqing 400715 |
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| Abstract: | Blood samples were collected from goats in Fengdu,Chongqing and the genome DNA was extracted from the whole blood.The 16S rRNA gene of Aeromonas salmonicida was PCR-amplified and two fragments about 1 500 bp in length were obtained,which were then cloned to pMD19-T vector and sequenced.The sequences were submitted to GenBank,the accession number being JF823571 and JF823572,respectively.Phylogenetic analysis of 16S rRNA sequences with 3 homologous sequences from A.caviae,3 from A.hydrophila,3 from A.media,4 ... |
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| Keywords: | goat Aeromonas salmonicida 16S rRNA cloning and sequence analysis |
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