| 梁平柚葡萄糖基转移酶基因的克隆及原核表达 |
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| 引用本文： | 马鑫,王斌,胡雨晴,李名扬,眭顺照.梁平柚葡萄糖基转移酶基因的克隆及原核表达[J].西南农业大学学报,2011,33(8). |
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| 作者姓名： | 马鑫 王斌 胡雨晴 李名扬 眭顺照 |
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| 作者单位： | 西南大学园艺园林学院重庆市花卉工程技术研究中心教育部南方山地园艺学重点实验室; |
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| 基金项目： | 教育部博士点基金资助项目(200806351015) |
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| 摘 要： | 基于GenBank公布的葡萄糖基转移酶(LGT)基因的核苷酸序列,设计并合成特异性引物,以梁平柚成年树幼叶总RNA为模板,通过RT-PCR方法扩增葡萄糖基转移酶基因,将该基因片段克隆到原核表达载体pET-28a(+)上,构建了该基因的融合表达载体pET28a-CmLGT,转化到大肠杆菌BL21(DE3)中.经1.0mmol/LIPTG诱导表达获得大小约为62Kd的目的融合蛋白.
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| 关 键 词： | 葡萄糖基转移酶 CmLGT基因 克隆 原核表达 |
Cloning and Prokaryotic Expression of Glucose Transferase Gene from Citrus maxima cv. 'Liangping' |
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| MA Xin,WANG Bin,HU Yu-qing,LI Ming-yang,SUI Shun-zhao School of Horticulture , L,scape,Southwest University,Chongqing Engineering Research Center for Floriculture,Key Laboratory of Horticulture Science for Southern Mountainous Regions,Ministry of Education,Chongqing ,China.Cloning and Prokaryotic Expression of Glucose Transferase Gene from Citrus maxima cv. 'Liangping'[J].Journal of Southwest Agricultural University,2011,33(8). |
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| Authors: | MA Xin WANG Bin HU Yu-qing LI Ming-yang SUI Shun-zhao School of Horticulture L scape Southwest University Chongqing Engineering Research Center for Floriculture Key Laboratory of Horticulture Science for Southern Mountainous Regions Ministry of Education Chongqing China |
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| Institution: | MA Xin,WANG Bin,HU Yu-qing,LI Ming-yang,SUI Shun-zhao School of Horticulture and Landscape,Southwest University,Chongqing Engineering Research Center for Floriculture,Key Laboratory of Horticulture Science for Southern Mountainous Regions,Ministry of Education,Chongqing 400716,China |
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| Abstract: | Based on the nucleotide sequences of glucose transferase gene(LGT)published on GenBank,specific primers were designed and synthesized.With the total RNA of the young leaves of an adult tree of Citrus maxima cv.Liangping as the template,the LGT gene was obtained by RT-PCR.The fragments of the LGT gene were cloned into the prokaryotic expression vector pET-28a(+) and the fusion expression vector pET28a-CmLGT was constructed,which was then transformed into E.coli BL21(DE3).The target fusion protein with a size... |
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| Keywords: | glucose transferase CmLGT gene cloning prokaryotic expression |
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