| 应用Dot—ELISA法检测肉品中沙门氏菌的研究 |
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| 引用本文： | 蔡家利,顾金泉.应用Dot—ELISA法检测肉品中沙门氏菌的研究[J].西南农业大学学报,1991,13(2):226-228. |
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| 作者姓名： | 蔡家利 顾金泉 |
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| 作者单位： | 西南农业大学牧医系
(蔡家利，王诗德)，重庆肉类联合加工厂
(顾金泉)，重庆肉类联合加工厂(张益) |
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| 摘 要： |
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| 关 键 词： | 猪肉 检测 Dot－ELISA 沙门氏菌 |
A STUDY OF ENZYME-LINKED IMMUNOSORBENT ASSAY(ELISA)FOR SALMONELLA DETECTION IN PORK |
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| Cai Jiali Wang Shide.A STUDY OF ENZYME-LINKED IMMUNOSORBENT ASSAY(ELISA)FOR SALMONELLA DETECTION IN PORK[J].Journal of Southwest Agricultural University,1991,13(2):226-228. |
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| Authors: | Cai Jiali Wang Shide |
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| Abstract: | Dot-ELISA is recommended for Salmonella detection in meat andother food products.One hundred samples of exported meat and another10 samples which had been exposed to Salmonella were detected with Dot-ELISA for the existence of Salmonella.The results in the former casewere all negative while in the latter they were all positive.Comparedwith the convemtional cultural identification test,Dot-ELISA appeared tohave better sensitivity,specificity and reproducibility.The results ofboth tests were virtually identical.of 312 samples in slaughter process,positive rate of the test was 19.23%.The coincident rate of the two testswas as high as 99.36%.No crossreaction was observed between Salmonellaand Staphylococcus aureus,Escherichia coli,Proteus,Pasteurella multio-cida and Erysipelothrix ehusipathiae.This technique proved to be simpleand convenient and took only 24 hours for the detection,3-4 days shorterthan required for the conventional test. |
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| Keywords: | Dot-ELISA salmonella pork detection |
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