| 矮牵牛的组织培养研究 |
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| 引用本文： | 瞿素萍.矮牵牛的组织培养研究[J].西南农业大学学报,2001,23(5):447-448. |
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| 作者姓名： | 瞿素萍 |
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| 作者单位： | 云南省农业科学院园艺所花卉研究中心，云南昆明650205 |
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| 基金项目： | 国家计委和云南省科委资助项目 |
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| 摘 要： | 矮牵牛以茎尖、茎段和叶片为外植体，以MS为基本培养基，添加不同的激素配比，在MS BA2mg/L NAA0.1mg/L上诱导形成的愈伤组织块，再移至MS BA1 mg/L NAA 0.1mg/L上分化形成苗，如此交替作用，可达到最高的繁殖倍数、诱导率和分化率，变异苗也最少，能取得最佳效果；生根适宜的培养基为NS NAA0.5mg/L和MS NAA0.3mg/L IAA 0.2mg/L。
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| 关 键 词： | 矮牵牛 组织培养 愈伤组织 诱导率 牵牛花 |
| 文章编号： | 1000-2642(2001)05-0447-02 |
| 修稿时间： | 2001年3月5日 |
TISSUE CULTURE OF COLORED COMMON CALLALILY (PETUNIA HYBRIDA) |
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| QU Su-ping.TISSUE CULTURE OF COLORED COMMON CALLALILY (PETUNIA HYBRIDA)[J].Journal of Southwest Agricultural University,2001,23(5):447-448. |
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| Authors: | QU Su-ping |
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| Abstract: | Shoot tips, stem segments and young leaves of Petunia hybrida were adopted as explants and cultured on MS medium supplemented with different combinations of plant growth regulators. Best results of callus induction were achieved on MS enriched with BA 2 mg/L and NAA 0.1 mg/L. The calli were then transferred onto MS supplemented with BA 1 mg/L and NAA 0.1 mg/L for shoot differentiation. Repeated operation of the two procedures gave the highest propagation coefficient, induction rate and differentiation percentage, and the least aberration. Optimum media were MS NAA 0.5 mg/L and MS NAA 0.3 mg/L IAA 0.2 mg/L. |
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| Keywords: | Petunia hybrida tissue culture callus induction rate |
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