重庆地区草地贪夜蛾肠道细菌的分离鉴定

为了解入侵重庆地区草地贪夜蛾(Spodoptera frugiperda)肠道所携带的细菌组成,以采集自巫溪、巫山地区玉米地里的草地贪夜蛾为材料,运用传统培养方法分离了其肠道优势细菌,并基于16S rDNA测序开展了优势菌的属水平鉴定,共获得了30个细菌分离株,经16S rDNA序列同源性分析,可归于5个属,分别为克雷伯氏菌属(Klebsiella)、不动杆菌属(Acinetobacter)、假单胞菌属(Pseudomonas)、肠杆菌属(Enterobacter)以及气单胞菌属(Aeromonas);其中,克雷伯氏菌属(Klebsiella)的丰度最高,占所有分离菌株的63%;假单胞属(Pseudomonas)和肠杆菌属(Enterobacter)菌株仅在巫山地区分离得到,气单胞菌属(Aeromonas)仅在巫溪地区分离得到.实验确定了入侵重庆地区草地贪夜蛾肠道优势细菌的种类及丰度,为后续研究草地贪夜蛾肠道微生物对宿主的生长发育以及迁飞等重要问题奠定了基础.

Isolation and Identification of Gut Bacteria of Spodoptera frugiperda That Migrated to Chongqing Area

In order to understand the composition of bacteria in the guts of fall armyworm (FAW, Spodoptera frugiperda) that migrated to Chongqing, the dominant bacteria from the pest samples collected from maize fields in Wushan and Wuxi of Chongqing were isolated with the traditional culture method and identified with 16S rDNA sequencing. A total of 30 isolates of gut bacteria were obtained, and were clustered in 16S rDNA homology analyses into 5 taxa, i.e. Klebsiella, Acinetobacter, Pseudomonas, Enterobacter and Aeromonas. Of the 5 genera identified, Klebsiella had the highest abundance, accounting for 63% of the total. Pseudomonas and Enterobacter were isolated only in pest samples of Wushan, while Aeromonas was only found in those of Wuxi. In conclusion, this study has identified the generic composition of the intestinal bacteria of S. frugiperda that has migrated to Chongqing and determined their abundance, which may serve as a basis for future work on the impact of the intestinal microbes on the growth and development and migration of S. frugiperda.