重庆地区取食高粱的草地贪夜蛾肠道细菌新分离株的鉴定

为充分认识入侵重庆地区草地贪夜蛾(Spodoptera frugiperda)的肠道微生物, 2019年7月该课题组再次在重庆江津地区高粱地采集了草地贪夜蛾幼虫,通过分离培养结合16S rDNA测序鉴定,从江津草地贪夜蛾幼虫肠道中共分离得到38个细菌分离株,归为9个属,分别为克雷伯氏菌属(Klebsiella)、肠球菌属(Enterococcus)、微球菌属(Micrococcus)、葡萄球菌属(Staphylococcus)、芽孢杆菌属(Bacillus)、不动杆菌属(Acinetobacter)、寡养单胞菌属(Stenotrophomonas)、假单胞菌属(Pseudomonas)和鞘脂杆菌属(Sphingobacterium).在课题组前期研究的基础上,新分离到了6个属,分别为克雷伯氏菌属(Klebsiella)、微球菌属(Micrococcus)、葡萄球菌属(Staphylococcus)、芽孢杆菌属(Bacillus)、寡养单胞菌属(Stenotrophomonas)和鞘脂杆菌属(Sphingobacterium).同时还对入侵重庆地区采食高粱和玉米的草地贪夜蛾幼虫肠道细菌分离株进行了比较分析,只在采食高粱的草地贪夜蛾幼虫肠道中发现了欧文氏菌属(Erwinia)、微球菌属(Micrococcus)、葡萄球菌属(Staphylococcus)、芽孢杆菌属(Bacillus)和寡养单胞菌属(Stenotrophomonas)的菌株,而气单胞菌属(Aeromonas)、短波单胞菌属(Brevundimonas)、金黄杆菌属(Chryseobacterium)和类香味菌属(Myroides)则只在采食玉米的草地贪夜蛾幼虫肠道中分离得到.通过对同一片江津高粱地的草地贪夜蛾和玉米黏虫(Mythimna separata)的肠道细菌分离株进行比较分析,只在草地贪夜蛾肠道中发现了微球菌属(Micrococcus)、欧文氏菌属(Erwinia)、假单胞菌属(Pseudomonas)、葡萄球菌属(Staphylococcus)和鞘脂杆菌属(Sphingobacterium),而金黄杆菌属(Chryseobacterium)只发现于玉米黏虫肠道中.

Identification of New Isolates of Gut Bacteria of Spodoptera frugiperda Feeding on Sorghum in Chongqing Area

In order to fully understand the intestinal microbes of Spodoptera frugiperda which migrated to Chongqing, our team recently collected again the S. frugiperda larvae feeding on sorghum in July of 2019. Through isolation, culture and 16S rDNA sequencing, we obtained 38 isolates of bacteria from the gut of S. frugiperda in sorghum fields in Jiangjin, which belonged to 9 genera, including Klebsiella, Enterococcus, Micrococcus, Staphylococcus, Bacillus, Acinetobacter, Stenotrophomonas, Pseudomonas and Sphingobacterium. Compared with our previous research, 6 unreported genera were identified, including Klebsiella, Micrococcus, Staphylococcus, Bacillus, Stenotrophomonas and Sphingobacterium. At the same time, we also conducted a comparative analysis of the intestinal bacteria isolates of S. frugiperda feeding on sorghum and on corn. Strains of Erwinia, Micrococcus, Staphylococcus, Bacillus and Stenotrophomonas were found only in the gut of S. frugiperda feeding on sorghum, and Aeromonas, Brevundimonas, Chryseobacterium and Myroides were isolated only from the S. frugiperda feeding on corn. A comparison of the larvae intestinal bacterial isolates of S. frugiperda and Mythimna separata in the same sorghum field in Jiangjin showed that Micrococcus, Erwinia, Pseudomonas, Staphylococcus and Sphingobacterium were found only in the gut of S. frugiperda, while Chryseobacterium was found only in the gut of M. separata.