| 11种野生苹果种质资源RAPD标记研究 |
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| 引用本文： | 周志钦,李育农.11种野生苹果种质资源RAPD标记研究[J].西南农业大学学报,1998,20(1):34-37. |
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| 作者姓名： | 周志钦 李育农 |
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| 作者单位： | 西南农业大学园艺系 |
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| 摘 要： | 利用Ｃ．Ｇ．Ａｌｐｈａ等的ＤＮＡ微量提取法，提取了山荆子ＭａｌｕｓｂａｃｃａｔａＢｏｒｋｈ．等１１个苹果属植物野生种类的ＤＮＡ，并用５对引物对所研究材料的ＲＡＰＤ标记进行了研究。探讨了叶片中富含多酚类化合物的苹果植物叶片ＤＮＡ的提取及纯化方法，并初步建立了野生苹果ＲＡＰＤ标记的ＰＣＲ反应条件。
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| 关 键 词： | 苹果属 野生果树 种质资源 RAPD标记 |
A PRELIMINARY STUDY ON RAPD MARKERS OF WILD SPECIES IN MALUS MILL. |
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| Zhou Zhiqin, Li Yunong, Wang Lichao.A PRELIMINARY STUDY ON RAPD MARKERS OF WILD SPECIES IN MALUS MILL.[J].Journal of Southwest Agricultural University,1998,20(1):34-37. |
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| Authors: | Zhou Zhiqin Li Yunong Wang Lichao |
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| Abstract: | The DNA of eleven wild species from Malus Mill. were extracted by using a new method developed by Christopher G.Alpha etc. Five pairs of primers of group P from Operon Company were used to establish the RAPD markers of the species. As a result, We got the RAPD markers of five out of 11 species, and established the reaction parameters for PCR of Malus wild species. The problems were discussed of isolating DNA from plant tissues rich in polyphenolics, like the leaves of Malus species. |
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| Keywords: | Malus wild fruittrees germ plasm resources /RAPD markers |
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