| PRRSVGP5基因的优化、表达及其表达产物的免疫学活性分析 |
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| 引用本文： | 黄园媛,邹灵秀,李能章,彭远义.PRRSVGP5基因的优化、表达及其表达产物的免疫学活性分析[J].西南农业大学学报,2012,34(1):001-005. |
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| 作者姓名： | 黄园媛 邹灵秀 李能章 彭远义 |
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| 作者单位： | 西南大学动物科技学院 |
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| 基金项目： | 重庆市自然科学基金重点项目(CSTC.2008BA1011) |
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| 摘 要： | PRRSV GP5基因含有一段信号肽及3个跨膜功能区,这些区域可以使翻译的GP5停留在内质网,从而抑制其表达.根据实验室分离测定的PRRSV T1株GP5基因序列进行优化设计,通过人工合成获得GP5-ShortDNA并构建表达载体pGEX-GP5,将其转入大肠杆菌BL21,经IPTG诱导表达获得约41kD的目的融合蛋白.通过Western-blot检测,结果显示该融合蛋白可与PRRSV阳性血清发生特异性反应,表明该蛋白具有免疫学活性.研究结果为PRRSV基因工程苗的研究奠定了基础,同时也为基因的优化提供了借鉴.
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| 关 键 词： | PRRSV GP5 基因合成 原核表达 免疫学活性 |
Optimization and Expression of GP5 Gene of PRRSV and Analysis of Immunological Activity of Its Expressed Products |
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| HUANG Yuan-yuan,ZOU Ling-xiu,LI Neng-zhang,PENG Yuan-yi.Optimization and Expression of GP5 Gene of PRRSV and Analysis of Immunological Activity of Its Expressed Products[J].Journal of Southwest Agricultural University,2012,34(1):001-005. |
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| Authors: | HUANG Yuan-yuan ZOU Ling-xiu LI Neng-zhang PENG Yuan-yi |
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| Institution: | School of Animal Science and Technology,Southwest University,Chongqing 400715,China |
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| Abstract: | GP5 gene of PRRSV contains a signal peptide and three transmembrane functional areas,and these sequences can make GP5 stay in the endoplasmic reticulum and inhibit its expression.In this report,the GP5 gene of PRRSV T1 strain separated and determined by our laboratory was optimized,GP5-ShortDNA was synthesized and the optimized gene was cloned into the expressive vector pGEX-GP5.The recombinant protein(about 41 kD) was expressed in E.coli BL21 after induction with IPTG.Western blot showed that the recombinant protein had immunological activity with the reference positive serum. |
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| Keywords: | PRRSV GP5 gene synthesis procaryotic expression immunological activity |
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