马缨杜鹃离体快繁体系的建立及优化

以马缨杜鹃种子为外植体,建立和优化了马缨杜鹃高效而稳定的离体快繁体系,并采用ISSR标记对试管苗的遗传稳定性进行分子检测.结果表明:种子在接种于1/4MS+1.0mg/L KT培养基上,萌发率在70%以上,丛生芽诱导以无菌苗带顶芽茎段为佳,最适培养基为WPM附加0.5mg/L TDZ和1.0mg/L IBA,丛芽伸长最适培养基为WPM+KT 0.5mg/L+GA 1.0mg/L.在1/2WPM+KT 0.5mg/L+NAA 1.0mg/L+IBA 2.0mg/L培养基上30d开始生根,生根率可达100%.90d后驯化移栽成活率在80%以上.采用20个ISSR引物扩增,对通过上述流程快繁的试管苗的体细胞无性系遗传变异进行分子检测,未检测到多态性带,表明该快繁体系能生产出遗传稳定性高的试管苗.

EstablishmentandOptimizationofinvitro MicropropagationofRhododendrondelavayiFranch

Using seeds of Rhododendron delavayi Franch as the explants,an efficient micropropagation system for this plant species was established and optimized,and ISSR markers were used to assess the genetic stability of the regenerated plantlets.The seeds germinated well in 1/4MS+1.0 mg/L KT and gave a germination rate of 70% or more.Shoot sections with a terminal bud were the best explants,and WPM(Woody Plant Medium)+KT 0.5 mg/L+GA 1.0 mg/L was the optimum medium for bud cluster induction.WPM+KT 0.5 mg/L+GA 1.0 mg/L was the optimum medium for the growth of the induced bud clusters.With the addition of 0.5 mg/L KT,1.0 mg/L NAA and 2.0 mg/L IBA,1/2WPM demonstrated the best root induction effect after 30 days,whose rooting rate was as high as 100%.After 90-day acclimatization,80% of the plantlets survived after transplantation.ISSR amplification with 20 primers was made to test the somaclonal variation of the plantlets and no polymorphic bands were detected,thus indicating that this micropropagation system could produce plantlets with high genetic stability.