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西瓜子叶组织培养中四倍体的产生及鉴定
引用本文:郭启高,宋明,杨天秀,梁国鲁.西瓜子叶组织培养中四倍体的产生及鉴定[J].西南农业大学学报,2000,22(4):298-300.
作者姓名:郭启高  宋明  杨天秀  梁国鲁
作者单位:西南农业大学园艺系,重庆
摘    要:5年二倍体西瓜品种子叶离体培养过程中,外植体分化频率、增殖系数、畸芽率及四倍体频率随基因型、外植体所处部位、激素浓度不同而存在着较大差异。用不定芽叶尖染色体计数可100%要出四倍体。试管苗快繁中四倍体对营养逆境有较强的适应性。

关 键 词:西瓜  组织培养  四倍体  子叶
文章编号:1000-2642(2000)04-0298-03
修稿时间:2000-01-03

REGENERATION AND IDENTIFICATION OF TETRAPLOIDS ORIGINATING FROM COTYLEDONS OF DIPLOID WATERMELON CULTURED 1N VITRO
GUO Qi-gao ,SONG Ming ,YANG Tian-xiu ,LIANG Guo-lu.REGENERATION AND IDENTIFICATION OF TETRAPLOIDS ORIGINATING FROM COTYLEDONS OF DIPLOID WATERMELON CULTURED 1N VITRO[J].Journal of Southwest Agricultural University,2000,22(4):298-300.
Authors:GUO Qi-gao  SONG Ming  YANG Tian-xiu  LIANG Guo-lu
Institution:GUO Qi-gao ,SONG Ming ,YANG Tian-xiu ,LIANG Guo-lu ;(Department of Horticulture, Southwest Agricultural University, Chongqing 400716, China)
Abstract:Experiments were made with cultured cotyledons of 5 diploid varieties of watermelon Citrullus lantus (Matsums. & Nakai)]. Differentiation frequency of the explants, mulfplicafon coefficient, malformed shoot percentage and frequency of tetraploids varied considrably with genotype, explants and hormone levels in the medium. All the tetraploids could be easily detected by examining the chromosome number in the apical cells of the adventitious shoots. Tetraploid plants showed better adaptation to nutrition stress in the culture medium than their diploid counterparts.
Keywords:watermelon  tissue culture  tetxaploid
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