川茶花品种间亲缘关系探讨

采用聚丙烯酰胺凝胶电泳方法,检测了川茶花19个品种叶片的过氧化物酶同工酶。结果表明,从19个品种中分离出20条酶带。其中Px2,3,10,17等4条酶带构成了川茶花的基本酶谱。按Px8,9,10,11等4条酶带的不同组合形式,将19个品种划分为5种同工酶表型;同一表型品种的花型、花色不一定相同,即同工酶表型与园艺分类存在着交叉现象,认为在川茶花的系统发育过程中,花型、花色的分化晚于同工酶表型分化,同工酶表型相同的品种间亲缘关系近,而花型、花色的差异不反应遗传差异。另外,在所测的19个品种之间,无两个完全相同的酶谱,这为川茶花品种的鉴别提供了依据。

A STUDY ON THE RELATIONSHIP AMONG DIFFERENT VARIETIES OF Camellia japonica L.THROUGH ISOZYME ANALYSIS

Polyacrylamide gel electrophoresis(PAGE)of the leaves of 19 varieties of Camellia japonica L.showed 20 separate bands,of which four bands,i.e.Px2,3,10 and 17,formed the basic isozyme zymograms of the species.Based on different combinations of another four bands,i.e.Px8,9,10 and 11,the varieties studied fell into 5 categories.Flowers of dif- ferent varieties in a category were not necessarily of the same type and colour,indicating certain overlapping between isozyme patterns and horticultural taxonomy.It is supposed that in the course of evolution of C.japonica,the differentiation of flower type and colour might have occurred later than that in isozyme patterns.Hence,varieties of the same isozyme category may be more closely related genetically while variations in flower type and colour do not necessarily reflect genetical differences.In addition,no identical zymograms were noticed in the 19 varieties,a fact which may provide a guide for identifica- tion of different varieties of C.japonica L.