油菜隐性核不育系从低芥向双低转育的技术

用双低品种对不育系转育测交后，在F2代分离出的不育株上再用其品种回交，然后在B1F2（回交种的F2）代群体中选可育株自交，自交株通过室内硫甙粗测分析淘汰种植B1F3的低硫株行，花期选择出现1／4不育株率的株系进行系内兄妹交，再种植鉴定，选50％左右不育株率的株系成对测交。用3年6代（含夏繁）育成了不育株率为51．8％、硫甙含量为31．2μmol/g的不育系－油研7号双低不育系。其选择群体在100－500株的范围，双低株系的入选株率在5％以上，不育系稳定的入选株系率可达60％左右。

Breeding Technique for Transforming Recessive GMS Lines from Low Erucic Acid into Double Low (Low Erucic Acid and Low Glucosinolate) in Rape

Youyan 7, a sterile line, with double low (low erucic acid andlow glucosinolate) and 51.8% of sterile plant rate and 31.2μmol of glucosinolate content was bred out for 3 years and 6 generations (contained reproduction in Summer) by a series of crosses and selection. Namely, after the sterile line was crossed and tested with the double-low variety, selected sterile individuals from its F2 population and backcrossed with its male parent, and in its B1F1 population, fertile plants was selected for self-pollination, and then took glucosinolate test to the self-bred and planted in row with the selected double low individuals of B1F3 generation. During the flowering period, selected lines with 1/4 of sterile plant rate and let it achieve sib mating within line. Then took field test to select lines with about 50% of sterile plant rate for test cross in pairs.