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| 马铃薯品种茎段愈伤组织诱导和植株再生的研究 | |
| 引用本文： | 邱礽,陶刚,朱英,黄永会,程仪琳,刘作易.马铃薯品种茎段愈伤组织诱导和植株再生的研究[J].贵州农业科学,2009,37(10). |
| 作者姓名： | 邱礽 陶刚 朱英 黄永会 程仪琳 刘作易 |
| 作者单位： | 1. 贵州大学,生命科学院,贵州,贵阳,550025;贵州省农业生物技术重点实验室,贵州,贵阳,550006 2. 贵州省农业生物技术重点实验室,贵州,贵阳,550006;贵州省植物保护研究所,贵州,贵阳,550006 3. 贵州省农业生物技术重点实验室,贵州,贵阳,550006;贵州省生物技术研究所,贵州,贵阳,550006 4. 贵州大学,生命科学院,贵州,贵阳,550025 5. 贵州省农业生物技术重点实验室,贵州,贵阳,550006 |
| 基金项目： | 973计划前期研究专项"利用基因沉默原理和基因删除技术创制抗病毒马铃薯新种质的研究"，贵州省科学技术重大专项"贵州马铃薯产业化关键技术研究与示范"，贵州省农业科学院重点专项"马铃薯抗病毒转基因表达载体构件及转基因技术研究" |
| 摘 要： | 以2个马铃薯品种夏坡蒂和费乌瑞它茎段为外植体,研究了不同植物激素对马铃薯愈伤组织诱导及其不定芽分化的影响.结果表明,夏坡蒂和费乌瑞它的茎段最佳愈伤诱导组织培养基分别为MS+6-BA2.0mg/L+NAA0.5mg/L+GA30.5mg/L和MS+ZT 2.0mg/L+NAA 0.1mg/L+GA30.5mg/L,愈伤率均可达到100%;夏坡蒂和费乌瑞它的不定芽最佳诱导培养基分别为MS+ZT 2.0 mg/L+NAA0.1mg/L+GA3 5mg/L和MS+6-BA 2.0mg/L+ZT 2.0mg/L+GA3 5mg/L,诱导率分别为52.38%和53.6%.结果为进一步转基因工作中高效植株的再生奠定了一定的基础. |
| 关 键 词： | 马铃薯 费乌瑞它 夏坡蒂 愈伤组织诱导 植株再生 |
Study on Callus Induction and Plantlet Regeneration from Stem Explants of Two Potato Varieties |
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| QIU Reng,TAO Gang,ZHU Ying,HUANG Yong-hui,CHENG Yi-lin,LIU Zuo-yi.Study on Callus Induction and Plantlet Regeneration from Stem Explants of Two Potato Varieties[J].Guizhou Agricultural Sciences,2009,37(10). | |
| Authors: | QIU Reng TAO Gang ZHU Ying HUANG Yong-hui CHENG Yi-lin LIU Zuo-yi |
| Abstract: | The stem explants of two potato varieties (Shepody and Favorite) were cultured on MS medium with different plant hormones to study the effects on callus induction and differentiation of adventitious buds. The results showed that the optimum medium for 100% callus induction rate of potato stem explants of Shepody and Favorite was MS+6-BA 2.0 mg/L+NAA 0.5 mg/L +GA_3 0.5 mg/L and MS+ZT 2.0 mg/L+NAA 0.1 mg/L+GA_30.5 mg/L, the optimum medium for differentiation of adventitious buds of Shepody and Favorite was MS+ZT 2.0 mg/L+NAA 0.1 mg/L+GA_3 5 mg/L and MS+6-BA 2.0 mg/L+ ZT 2.0 mg/L+GA_3 5 mg/L and their differentiation rates of adventitious buds reached 52.38% (Shepody) and 53.6% (Favorite) respectively. The results lay a certain foundation for further efficient transgenic plant regeneration. |
| Keywords: | potato Favorite Shepody callus induction plantlet regeneration |
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