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| 绵羊精原干细胞移植受体内源性生殖细胞的消除研究 | |
| 引用本文： | 窦红伟,赵兴绪,马月辉,冯书堂,员新旭,曹文广.绵羊精原干细胞移植受体内源性生殖细胞的消除研究[J].贵州农业科学,2009,37(3). |
| 作者姓名： | 窦红伟 赵兴绪 马月辉 冯书堂 员新旭 曹文广 |
| 作者单位： | 1. 甘肃农业大学,动物医学院,甘肃,兰州,730070;中国农业科学院,北京畜牧兽医研究所,北京100193 2. 甘肃农业大学,动物医学院,甘肃,兰州,730070 3. 中国农业科学院,北京畜牧兽医研究所,北京100193 |
| 基金项目： | 中国农业科学院杰出回国人才资金资助项目，北京畜牧兽药研究所杰出出国人才基金 |
| 摘 要： | 试验拟建立绵羊精原干细胞移植受体内源性生殖细胞的消除技术.将8只5月龄小尾寒羊公羊随机分为4组,各组第2次处理后3周,公羊睾丸活体采样,石蜡切片、HE染色验证处理效果.结果表明,白消安处理公羊可以完全消除内源性精原干细胞和其他生殖细胞.LHRH、LH疫苗处理的公羊,只能使部分精子发生丧失和生殖细胞消除.白消安是精原干细胞移植受体绵羊内源性生殖细胞最有效的消除技术. |
| 关 键 词： | 绵羊 精原干细胞 生殖细胞 白消安 免疫 |
Depleting Endogenous Germ Cells for Transplantation Recipients of Spermatogonial Stem Cells in Ovine |
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| DOU Hong-wei,ZHAO Xing-xu,MA Yue-hui,FENG Shu-tang,YUAN Xin-xu,CAO Wen-guang.Depleting Endogenous Germ Cells for Transplantation Recipients of Spermatogonial Stem Cells in Ovine[J].Guizhou Agricultural Sciences,2009,37(3). | |
| Authors: | DOU Hong-wei ZHAO Xing-xu MA Yue-hui FENG Shu-tang YUAN Xin-xu CAO Wen-guang |
| Institution: | 1.College of Veterinary Medicine;Gansu Agricultural University;Lanzhou;Gansu 730070;2.Institute of Animal Science;Chinese Academy of Agricultural Science;Beijing 100193;China |
| Abstract: | In order to establish the technique of depleting endogenous germ cells for transplantation recipients of spermatogonial stem cells in ovine,eight of 5-month-old small-tail Han rams were randomly divided into four experimental groups to test.After three weeks from the second treatment,the samples of testes are obtained in vivo.The testes samples were embedded in paraffin,section cut,stained with hematoxylin and eosin,and observed under a microscope.The results show that the endogenous spermatogonial stem cel... |
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