| 天蓝刺头组织培养及无性系建立 |
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| 引用本文： | 赵娜,冯家祺,王羽毛,徐娜,姜长阳.天蓝刺头组织培养及无性系建立[J].贵州农业科学,2009,37(6). |
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| 作者姓名： | 赵娜 冯家祺 王羽毛 徐娜 姜长阳 |
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| 作者单位： | 辽宁师范大学,生命科学学院,辽宁,大连,116029 |
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| 摘 要： | 以生长旺盛的天蓝刺头生长点为外植体,以附加不同浓度的BA、IAA、IBA、NAA、2,4-D、GA的1/2 MS或1/3 MS为培养基,进行了天蓝刺头生长点生长、不定芽分化、试管苗生根、试管苗移栽与扦插和试管苗移植的研究.结果表明:1/2MS+BA 0.2 mg/L+NAA 0.1 mg/L是芽生长的理想培养基;1/2MS+AgNO31.2 mg/L+BA 0.9 mg/L+NAA 0.1 mg/L是天蓝刺头分化培养的理想培养基;1/3MS+IAA 0.3 mg/L是天蓝刺头试管苗生根培养的理想培养基;炉灰渣和河沙是试管苗移栽和扦插的理想基质;移植于山坡上的试管苗生长旺盛,根系发达.
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| 关 键 词： | 天蓝刺头 无性系 快速繁殖 |
Tissue Culture of Echinops dissectus |
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| ZHAO Na,FENG Jia-qi,WANG Yu-mao,XU Na,JIANG Chang-yang.Tissue Culture of Echinops dissectus[J].Guizhou Agricultural Sciences,2009,37(6). |
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| Authors: | ZHAO Na FENG Jia-qi WANG Yu-mao XU Na JIANG Chang-yang |
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| Institution: | College of Life Science;Liaoning Normal University;Dalian;Liaoning 116029;China |
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| Abstract: | The growing points of Echinops dissectus were cultured on the 1/2 MS or 1/3 MS medium supplemented with different concentrations of BA,IAA,IBA,NAA,2,4-D and GA for growth of callus induction,bud differentiation and rooting.The results showed that the optimum medium for callus induction,bud differentiation and rooting is 1/2 MS+BA 0.2 mg/L+NAA 0.1 mg/L,MS+ AgNO3 1.2 mg/L+ BA 0.9 mg/L+NAA 0.1 mg/L and 1/3 MS+IAA 0.3 mg/L respectively.The optimum substrates for plantlet transplanting are slag and river sand.Th... |
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| Keywords: | Echinops dissectus clone rapid propagation |
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