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| 1株产几丁质酶绿僵菌的筛选及其产酶特性 | |
| 引用本文： | 王子坚,曾巧英,殷宏,刘志杰,任巧云,罗建勋.1株产几丁质酶绿僵菌的筛选及其产酶特性[J].贵州农业科学,2010,38(3). |
| 作者姓名： | 王子坚 曾巧英 殷宏 刘志杰 任巧云 罗建勋 |
| 作者单位： | 1. 甘肃农业大学,动物医学院,甘肃兰州730070;中国农业科学院,兰州兽医研究所,家畜疫病病原生物学国家重点实验室,甘肃省动物寄生虫病重点实验室,甘肃,兰州730046 2. 甘肃农业大学,动物医学院,甘肃兰州730070 3. 中国农业科学院,兰州兽医研究所,家畜疫病病原生物学国家重点实验室,甘肃省动物寄生虫病重点实验室,甘肃,兰州730046 |
| 基金项目： | 国家支撑计划项目“主要动物源性人畜共患细菌和寄生虫病防控生物新制剂与开发”(2007BAD40B06);;国家高技术研究发展计划(863计划)重点项目“硬蜱基因工程疫苗的研究”(2006AA10A207) |
| 摘 要： | 为了提高绿僵菌产几丁质酶的酶活力,在自然条件下,从患病蜱体表分离到10株绿僵菌与8株白僵菌,通过筛选,得到1株绿僵菌Ma002。对该菌液体深层发酵产几丁质酶进行了测定,结果表明:几丁质酶的最佳产酶条件:碳源为胶体几丁质,氮源为蛋白胨,接种种龄为24h,以瓶体积的12%为装基量,在温度26℃,起始pH值7.5～8.0,搅拌速度180r/min,产酶时程96h,酶活力可达2.63U/mL,比原基础培养基上的酶活力(1.67U/mL)提高57%。 |
| 关 键 词： | 几丁质酶 酶活力 发酵 |
Screening of Metarhizium anisopliae Produced Chitinase and Its Enzyme-Producing Characteristic |
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| WANG Zi-jian,ZENG Qiao-ying,YIN Hong,LIU Zhi-jie,REN Qiao-yun,LUO Jian-xun.Screening of Metarhizium anisopliae Produced Chitinase and Its Enzyme-Producing Characteristic[J].Guizhou Agricultural Sciences,2010,38(3). | |
| Authors: | WANG Zi-jian ZENG Qiao-ying YIN Hong LIU Zhi-jie REN Qiao-yun LUO Jian-xun |
| Institution: | 1.College of Veterinary Medicine;Gansu Agricultural University;Lanzhou;Gansu 730030;2.Lanzhou Veterinary Research Institute;State Key Laboratory of Veterinary Etiological Biology;Key Laboratory of Veterinary Parasitology of Gansu Province;Gansu 730046;China |
| Abstract: | 10 isolates of Metarhizium anisopliae and 8 isolates of Beauveria bassiana were isolated from dead body of tick in nature conditions to increase the chitinase-producing ability of M. anisopliae. One strain of M. anisopliae Ma002 was screened out. The chitinase produced from submerged fermentation of this strain was determined. The results showed that the optimal fermentation conditions for enzyme-producing were as following:colloid chitin as carbon source,peptone as nitrogen source,age to be inoculated 24 h... |
| Keywords: | chitinase enzyme activity fermentation |
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