| 噻苯隆对月季芽增殖及月季叶片愈伤诱导的影响 |
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| 引用本文： | 于相丽,秦磊,余时超.噻苯隆对月季芽增殖及月季叶片愈伤诱导的影响[J].贵州农业科学,2012,40(1):28-30. |
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| 作者姓名： | 于相丽 秦磊 余时超 |
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| 作者单位： | 洛阳师范学院,河南洛阳,471022 |
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| 基金项目： | 河南省教育厅自然科学研究计划项目，洛阳师范学院青年基金 |
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| 摘 要： | 为研究噻苯隆( TDZ)对月季的影响,以皇家巴西诺月季的茎段和叶片为外植体,MS为基本培养基,添加一定浓度的细胞分裂素和生长素及不同浓度的TDZ,对其芽增殖和叶片愈伤组织诱导进行了研究.结果表明:促进侧芽增殖的最佳培养基为MS+NAA 0.lmg/L+6BA1.0mg/L+TDZ 2.0mg/L,诱导月季叶片形成愈伤的最佳培养基为MS+NAA 0.5mg/L+2,4D5.0 mg/L+ TDZ 1.0mg/L,0.5 cm×1.0 cm的切块有利于愈伤组织的产生.
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| 关 键 词： | 月季 噻苯隆(TDZ) 芽增殖 愈伤组织诱导 |
Influence of TDZ on Bud Propagation and Leaf Callus Induction in Rose |
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| YU Xiang li
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QIN Lei
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YU Shi-chao.Influence of TDZ on Bud Propagation and Leaf Callus Induction in Rose[J].Guizhou Agricultural Sciences,2012,40(1):28-30. |
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| Authors: | YU Xiang li QIN Lei YU Shi-chao |
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| Institution: | (Luoyang Normal University,Luoyang,Henan 471022,China) |
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| Abstract: | The stem segments and leaves of rose were cultured on MS medium with cytokinin+ auxin+TDZ of different concentration to study the effects of TDZ on bud propagation and leaf callus induction.The results showed that the optimal medium for lateral bud propagation and leaf callus induction was MS+NAA 0.1 mg/L+6-BA 1.0 mg/L+TDZ 2 mg/L and MS+NAA 0.5 mg/L+2,4-D 5.0 mg/ L+TDZ 1.0 mg/ L respectively.The optimal leaf size for callus induction was 0.5 cm×1.0 cm. |
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| Keywords: | rose TDZ bud propagation callus induction |
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