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| 培养基成分对黔白大白菜杂交种小孢子胚诱导的影响 | |
| 引用本文： | 赵大芹,陶莲,张朝君,毛堂芬,徐涵.培养基成分对黔白大白菜杂交种小孢子胚诱导的影响[J].贵州农业科学,2007,35(6):24-25,29. |
| 作者姓名： | 赵大芹 陶莲 张朝君 毛堂芬 徐涵 |
| 作者单位： | 1. 贵州省园艺研究所,贵州省园艺工程技术研究中心,贵州,贵阳,550006 2. 贵州省农业科学院,生物技术研究所,贵州,贵阳,550006 3. 法国图卢兹综合科学研究所,图卢兹,31300 |
| 基金项目： | 贵州省农业动植物育种专项[黔农育专字(2005)029] |
| 摘 要： | 以2个黔白大白菜杂交组合为材料,进行游离小孢子培养,探讨了培养基成分对小孢子成胚率的影响。结果表明:NLN培养基添加13%蔗糖的培养效果最好;添加0.05 mg/L 6-BA和0.10 mg/L NAA的培养基显著提高了小孢子胚的诱导率和成熟胚的比例;NLN-13液体培养基中添加适量活性碳可明显促进小孢子胚状体的形成,对胚胎发育的同步性也有很好的作用。 |
| 关 键 词： | 大白菜 小孢子培养 培养基成分 胚状体 诱导率 |
| 文章编号： | 1001-3601（2007）06-0365-0024-02 |
| 收稿时间： | 2007-07-11 |
| 修稿时间： | 2007-10-14 |
The Effect of Media Composition on Embryoid Differentiation Rate of Microspores of Qianbai Chinese Cabbage Hybrid Combinations |
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| ZHAO Daqin,TAO Lian,ZHANG Chaojun,MAO Tangfen,XU Han.The Effect of Media Composition on Embryoid Differentiation Rate of Microspores of Qianbai Chinese Cabbage Hybrid Combinations[J].Guizhou Agricultural Sciences,2007,35(6):24-25,29. | |
| Authors: | ZHAO Daqin TAO Lian ZHANG Chaojun MAO Tangfen XU Han |
| Abstract: | The microspores from two hybrid combinations of Qianbai Chinese cabbage were cultured to investigate the effect of media composition on the embryoid differentiation rate.The results showed that the best induced effect was from NLN media 13% sucrose,the NLN media 0.05 mg/L 6-BA 0.10 mg/L NAA significantly improved the embryoid induced rate and ratio of mature embryos and NLN-13 liquid media rational active carbon obviously promoted the embryoid formation and development. |
| Keywords: | Chinese cabbage microspore culture media composition embryoid induced rate |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |