48份糯玉米自交系遗传多样性的SSR标记

为分析48份糯玉米自交系的遗传多样性及其之间的遗传关系,利用总DNA提取方法和微卫星分子标记(SSR)技术,取均匀分布各染色体的45对SSR引物对样品进行PCR扩增分析,筛选出23对扩增带型稳定的SSR引物,从供试材料中检测出141个等位基因变异,每对引物检测等位基因2~14个,平均5.48个。SSR引物的PIC介于0.43~0.89,平均多态性信息量为0.66。UPGMA方法聚类分析表明,供试材料间遗传距离变幅为0.26~0.72,平均值为0.49。SSR标记能将全部自交系区分开来,48份糯玉米自交系可分为六类。

Genetic Diversity of 48 Glutinous Maize Inbred Lines

The genetic diversity of 48 glutinous maize inbred lines and the genetic relationship between these lines were analyzed by the SSR molecular marker and total DNA extraction method. 23 pairs of stable SSR primers with the amplification band pattern were amplified from 45 pairs of SSR primers of samples. 141 alleles were detected from 48 tested inbred lines and 2～4 alleles with average 5.48 alleles were detected from a pair of primer. The polymorphism information content and mean polymorphism information content of each SSR primer was 0.43～0.89 and 0.66. The results from UPGMA clustering analysis showed that the genetic distance between tested lines was 0.26～0.72 and its mean value was 0.49.48 The glutinous maize inbred lines can be divided into six groups, which can provide a reference for its breeding.