| Alcalase碱性蛋白酶制备蚯蚓肽的酶解参数研究 |
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| 引用本文： | 覃麟,施晓丽,夏先林.Alcalase碱性蛋白酶制备蚯蚓肽的酶解参数研究[J].贵州农业科学,2010,38(8). |
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| 作者姓名： | 覃麟 施晓丽 夏先林 |
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| 作者单位： | 贵州大学,动物科学学院,贵州,贵阳,550025 |
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| 基金项目： | 贵州大学研究生创新基金项目"利用猪粪养殖蚯蚓与蚯蚓小肽加工利用技术开发研究"，贵州省农业攻关计划项目"无公害饲料添加剂的研制与产业化示范" |
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| 摘 要： | 为解决畜禽养殖污染问题及开发蛋白质饲料资源提供理论依据,以Alcalase碱性蛋白酶为工具,采用单因素(温度、酶解时间、pH值、酶用量及底物浓度比)试验考察了蚯蚓蛋白酶的酶解效果,再通过正交试验优化酶解参数.结果表明:1)单因素试验:蚯蚓蛋白水解度随酶解时间的延长而呈上升趋势,5 h后上升趋于平缓;随着温度的升高和pH值的增大均呈先上升后下降的趋势,60℃和pH值达8.0时达最大值;随酶用量的增加和底物浓度的增大均呈先上升后下降的趋势,酶用量超过4%和底物浓度达6%后开始下降.正交试验:酶解温度、时间、pH和加酶量对蚯蚓蛋白的酶解度有显著或极显著的影响,4个因素作用的主次顺序为:酶用量＞温度＞pH＞时间;最优组合为温度65℃、时间4 h、pH值8.0、加酶量5%.Alcalase碱性蛋白酶制备蚯蚓小肽的最佳工艺参数为底物浓度6%、温度65℃、时间4 h、pH值8.0、加酶量5%.
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| 关 键 词： | 蚯蚓 蚯蚓肽 酶水解 |
Study on Enzymatic Technology of Earthworm Peptides Produced by Alcalase |
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| QIN Lin,SHI Xiao-li,XIA Xian-lin.Study on Enzymatic Technology of Earthworm Peptides Produced by Alcalase[J].Guizhou Agricultural Sciences,2010,38(8). |
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| Authors: | QIN Lin SHI Xiao-li XIA Xian-lin |
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| Institution: | QIN Lin,SHI Xiao-li,XIA Xian-lin(College of Animal Science,Guizhou University,Guiyang,Guizhou 550025,China) |
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| Abstract: | Enzymatic hydrolysis effect of earthworm proteinase was determined using Alcalase as the tool based on different single factor tests,which included temperature,hydrolysis time,pH,enzyme dosage and substrate concentration,and the enzymatic parameters were optimized by the orthogonal test to provide theoretical basis for solving breeding pollution and developing protein feed resource.The results showed that the hydrolysis degree of earthworm protein raised with prolong of hydrolysis time and the ascend trend ... |
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| Keywords: | Alcalase |
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